Differential Gene Expression and Protein Phosphorylation as Factors Regulating the State of the Arabidopsis SNX1 Protein Complexes in Response to Environmental Stimuli.
Differential Gene Expression and Protein Phosphorylation as Factors Regulating the State of the Arabidopsis SNX1 Protein Complexes in Response to Environmental Stimuli.
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DOI：
10.3389/fpls.2016.01456
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发表时间：
2016
影响因子：
5.6
通讯作者：
Ivanov R
中科院分区：
文献类型：
--
作者：
Brumbarova T;Ivanov R
内质体膜蛋白的再循环对细胞运输和信号传递过程的调节有重要作用。拟南芥分拣Nexin(SNX)蛋白家族的成员被证明在响应外界挑战时介导转运蛋白的内体恢复。我们的目的是了解外界刺激影响根中SNX1活性的可能途径。已知有几种蛋白质通过直接的蛋白质-蛋白质相互作用参与SNX1的功能。因此，我们编制了所有已知与SNX1物理上相互作用的拟南芥蛋白质清单，并利用可用的基因表达和蛋白质组数据来全面分析这种相互作用组的转录和转录后调控。编码SNX1相互作用伙伴的基因表现出不同的表达模式，其中一些基因，如FAB1A，统一表达，而另一些基因，如MC9和BLOS1，在特定的根区和细胞类型中表达。在已知诱导SNX1依赖反应的胁迫条件下，编码SNX1相互作用蛋白的两个基因MC9和NHX6显示出主要的基因表达变化。我们还可以观察到缺铁条件下SNX1的区带特异性转录变化，这与SNX1蛋白所描述的作用是一致的。这表明，根中潜在的含SNX1的蛋白质复合体的组成是细胞特有的，可能会对外界刺激做出反应而重新调整。在转录后修饰的水平上，我们观察到SNX1、FAB1A和CLASP的磷酸化状态随应激而变化。有趣的是，影响SNX1相互作用的磷酸化事件发生在一种与转录调控很大程度上互补的模式中。我们的分析表明，转录和转录后调控在外部应激下SNX1介导的内体循环中发挥着不同的作用。
Endosomal recycling of plasma membrane proteins contributes significantly to the regulation of cellular transport and signaling processes. Members of the Arabidopsis (Arabidopsis thaliana) SORTING NEXIN (SNX) protein family were shown to mediate the endosomal retrieval of transporter proteins in response to external challenges. Our aim is to understand the possible ways through which external stimuli influence the activity of SNX1 in the root. Several proteins are known to contribute to the function of SNX1 through direct protein–protein interaction. We, therefore, compiled a list of all Arabidopsis proteins known to physically interact with SNX1 and employed available gene expression and proteomic data for a comprehensive analysis of the transcriptional and post-transcriptional regulation of this interactome. The genes encoding SNX1-interaction partners showed distinct expression patterns with some, like FAB1A, being uniformly expressed, while others, like MC9 and BLOS1, were expressed in specific root zones and cell types. Under stress conditions known to induce SNX1-dependent responses, two genes encoding SNX1-interacting proteins, MC9 and NHX6, showed major gene-expression variations. We could also observe zone-specific transcriptional changes of SNX1 under iron deficiency, which are consistent with the described role of the SNX1 protein. This suggests that the composition of potential SNX1-containing protein complexes in roots is cell-specific and may be readjusted in response to external stimuli. On the level of post-transcriptional modifications, we observed stress-dependent changes in the phosphorylation status of SNX1, FAB1A, and CLASP. Interestingly, the phosphorylation events affecting SNX1 interactors occur in a pattern which is largely complementary to transcriptional regulation. Our analysis shows that transcriptional and post-transcriptional regulation play distinct roles in SNX1-mediated endosomal recycling under external stress.
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