Accelerated neuritogenesis and maturation of primary spinal motor neurons in response to nanofibers.
Accelerated neuritogenesis and maturation of primary spinal motor neurons in response to nanofibers.
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DOI：
10.1002/dneu.20792
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发表时间：
2010-07
影响因子：
3
通讯作者：
Corey, Joseph M.
中科院分区：
文献类型：
--
作者：
Gertz, Caitlyn C.;Leach, Michelle K.;Birrell, Lisa K.;Martin, David C.;Feldman, Eva L.;Corey, Joseph M.
神经元的发生、神经元的极性形成以及轴突和树突的成熟都受到细胞外生化成分的强烈影响。本研究的目的是阐明聚乳酸(PLLA)电纺纤维形态对初级运动神经元发育的影响，因为运动轴突在中枢神经系统中的再生极其有限，并可能受益于合成支架的实施以促进再生长。在这项分析中，我们发现，与平板聚合物膜和玻璃对照组相比，排列和随机取向的亚微米纤维都显著加速了单个培养运动神经元的神经元发生和极性形成的过程，这可能是由于纤维上观察到的限制片状脂膜的形成。相反，在体外培养2天后，树突的成熟和胞体在纤维基质上的铺展受到抑制。本研究首次探讨了电纺纤维形态对运动神经元发生和极性形成的影响。排列的纳米纤维被证明影响运动神经元发育的方向性和时间，为电纺支架在神经再生应用中的有效使用提供了进一步的证据。
Neuritogenesis, neuronal polarity formation, and maturation of axons and dendrites are strongly influenced by both biochemical and topographical extracellular components. The aim of this study was to elucidate the effects of polylactic acid (PLLA) electrospun fiber topography on primary motor neuron development, since regeneration of motor axons is extremely limited in the central nervous system and could potentially benefit from the implementation of a synthetic scaffold to encourage re-growth. In this analysis, we found that both aligned and randomly-oriented submicron fibers significantly accelerated the processes of neuritogenesis and polarity formation of individual cultured motor neurons compared to flat polymer films and glass controls, likely due to restricted lamellipodia formation observed on fibers. In contrast, dendritic maturation and soma spreading were inhibited on fiber substrates after 2 days in vitro. This study is the first to examine the effects of electrospun fiber topography on motor neuron neuritogenesis and polarity formation. Aligned nanofibers were shown to affect the directionality and timing of motor neuron development, providing further evidence for the effective use of electrospun scaffolds in neural regeneration applications.
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