Arbuscular mycorrhizal fungal spores host bacteria that affect nutrient biodynamics and biocontrol of soil-borne plant pathogens.
Arbuscular mycorrhizal fungal spores host bacteria that affect nutrient biodynamics and biocontrol of soil-borne plant pathogens.
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DOI：
10.1242/bio.2011014
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发表时间：
2012-01-15
期刊：
影响因子：
2.4
通讯作者：
Ishii T
中科院分区：
文献类型：
--
作者：
Cruz AF;Ishii T
从丛枝菌根真菌（AMF）孢子中分离细菌并对其进行鉴定。我们将这些细菌命名为“可能的内细菌”（PE）。使用皮下注射针（直径，200 μm）从约500个Gigaspora margarita（Becker和Hall）孢子中分离出3个细菌菌株。 通过形态学方法和核糖体基因序列鉴定细菌为芽孢杆菌属（KTCIGM 01）、苏云金芽孢杆菌（KTCIGM 02）和根际类芽孢杆菌（KTCIGM 03）。我们评估了这些可能的内生细菌对土传植物病原菌（SBPP）尖孢镰刀菌f。莴苣孢霉MAFF 744088、玫瑰茄病菌、立枯丝核菌MAFF 237426和终极腐霉NBRC 100123。我们还测试了这些可能的内细菌是否影响磷溶解，乙烯生产，固氮酶活性（NA），和刺激AMF菌丝生长。此外，使用原位扫描电子显微镜（SEM）（Quanta 250 FEG; FEI Co.，日本）。在菌丝和孢子表面可检测到类似生物膜的细菌聚集体。我们证明，使用浸提与一个无菌针，它是可能的，以隔离AMF相关的细菌物种，可能来自内部的真菌孢子。
The aim of this research was to isolate and characterize bacteria from spores of arbuscular mycorrhizal fungi (AMF). We designated these bacteria ‘probable endobacteria’ (PE). Three bacterial strains were isolated from approximately 500 spores of Gigaspora margarita (Becker and Hall) using a hypodermic needle (diameter, 200 μm). The bacteria were identified by morphological methods and on the basis of ribosomal gene sequences as Bacillus sp. (KTCIGM01), Bacillus thuringiensis (KTCIGM02), and Paenibacillus rhizospherae (KTCIGM03). We evaluated the effect of these probable endobacteria on antagonistic activity to the soil-borne plant pathogens (SBPPs) Fusarium oxysporum f. sp. lactucae MAFF 744088, Rosellinia necatrix, Rhizoctonia solani MAFF 237426, and Pythium ultimum NBRC 100123. We also tested whether these probable endobacteria affected phosphorus solubilization, ethylene production, nitrogenase activity (NA), and stimulation of AMF hyphal growth. In addition, fresh samples of spores and hyphae were photographed using an in situ scanning electron microscope (SEM) (Quanta 250FEG; FEI Co., Japan). Bacterial aggregates (BAs), structures similar to biofilms, could be detected on the surface of hyphae and spores. We demonstrate that using extraction with an ultrathin needle, it is possible to isolate AMF-associated bacterial species that are likely derived from inside the fungal spores.
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影响因子：
4.4
作者：
Bianciotto, V;Bandi, C;Bonfante, P
通讯作者：
Bonfante, P
影响因子：
4.9
作者：
LI, CY;MASSICOTE, HB;MOORE, LVH
通讯作者：
MOORE, LVH
影响因子：
4.4
作者：
Bertaux, J;Schmid, M;Frey-Mett, P
通讯作者：
Frey-Mett, P
DOI：
10.1139/b90-280
发表时间：
1990-10-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
DUPONNOIS, R;GARBAYE, J
通讯作者：
GARBAYE, J
影响因子：
9.4
作者：
BONFANTE, P;BALESTRINI, R;MENDGEN, K
通讯作者：
MENDGEN, K