Reduction of hydrogen peroxide accumulation and toxicity by a catalase from Mycoplasma iowae.
Reduction of hydrogen peroxide accumulation and toxicity by a catalase from Mycoplasma iowae.
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DOI：
10.1371/journal.pone.0105188
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Balish MF
中科院分区：
文献类型：
--
作者：
Pritchard RE;Prassinos AJ;Osborne JD;Raviv Z;Balish MF
爱荷华州支原体是一种公认的禽类病原体，可以感染和损害全身的许多部位。支原体细胞损伤的一种潜在介质是通过甘油分解代谢途径产生 H2O2，该途径的基因广泛存在于许多支原体物种中。先前对 M. iowae 血清变型 I 菌株 695 的测序表明，不仅存在通过甘油分解代谢产生 H2O2 的基因，而且还存在第一个记录的支原体过氧化氢酶基因，该酶可降解 H2O2。为了测试 M. iowae 过氧化氢酶降解 H2O2 的活性，我们研究了 M. iowae 血清型 K 菌株 DK-CPA 的过氧化氢酶活性和 H2O2 积累，我们对其基因组进行了测序，以及产生 H2O2 的物种鸡毒支原体菌株，通过用 M. iowae 推定的过氧化氢酶基因 katE 转化来生产 M. iowae 过氧化氢酶。还使用秀丽隐杆线虫毒性测定法分析了 H2O2 介导的 M. iowae 血清型 K 和产生过氧化氢酶的鸡毒支原体转化体的毒力，该测定法以前从未与支原体结合使用。我们发现 M. iowae katE 编码一种活性过氧化氢酶，当该酶在鸡毒支原体中表达时，可以减少产生的 H2O2 量以及甘油存在下对秀丽隐杆线虫的损害程度。因此，甘油分解代谢基因的存在与支原体利用 H2O2 作为毒力因子之间的相关性可能不是绝对的。
Mycoplasma iowae is a well-established avian pathogen that can infect and damage many sites throughout the body. One potential mediator of cellular damage by mycoplasmas is the production of H2O2 via a glycerol catabolic pathway whose genes are widespread amongst many mycoplasma species. Previous sequencing of M. iowae serovar I strain 695 revealed the presence of not only genes for H2O2 production through glycerol catabolism but also the first documented mycoplasma gene for catalase, which degrades H2O2. To test the activity of M. iowae catalase in degrading H2O2, we studied catalase activity and H2O2 accumulation by both M. iowae serovar K strain DK-CPA, whose genome we sequenced, and strains of the H2O2-producing species Mycoplasma gallisepticum engineered to produce M. iowae catalase by transformation with the M. iowae putative catalase gene, katE. H2O2-mediated virulence by M. iowae serovar K and catalase-producing M. gallisepticum transformants were also analyzed using a Caenorhabditis elegans toxicity assay, which has never previously been used in conjunction with mycoplasmas. We found that M. iowae katE encodes an active catalase that, when expressed in M. gallisepticum, reduces both the amount of H2O2 produced and the amount of damage to C. elegans in the presence of glycerol. Therefore, the correlation between the presence of glycerol catabolism genes and the use of H2O2 as a virulence factor by mycoplasmas might not be absolute.
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影响因子：
3.1
作者：
BANAI, M;KAHANE, I;BREDT, W
通讯作者：
BREDT, W
DOI：
10.1128/aem.21.4.594-599.1971
发表时间：
1971-01-01
期刊：
APPLIED MICROBIOLOGY
影响因子：
--
作者：
BARBER, TL;FABRICANT, J
通讯作者：
FABRICANT, J
影响因子：
3.2
作者：
Dhandayuthapani, S;Blaylock, MW;Baseman, JB
通讯作者：
Baseman, JB
影响因子：
3.1
作者：
Couillault, C;Ewbank, JJ
通讯作者：
Ewbank, JJ
影响因子：
2.8
作者：
Ley, David H.;Marusak, Rosemary A.;Fletcher, Oscar J.
通讯作者：
Fletcher, Oscar J.