Distinct modulated pupil function system for real-time imaging of living cells.
Distinct modulated pupil function system for real-time imaging of living cells.
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DOI：
10.1371/journal.pone.0044028
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yanagida T
中科院分区：
文献类型：
--
作者：
Watanabe TM;Tsukasaki Y;Fujita H;Ichimura T;Saitoh T;Akira S;Yanagida T
在过去的几十年里，光学显微镜是细胞生物学中最有贡献的工具之一。到目前为止，已经发展了许多具有各种功能的显微技术，如相差显微镜、差分干涉对比(DIC)显微镜、共聚焦显微镜、双光子显微镜、超分辨显微镜等。然而，负责实验的人必须从几种显微技术中选择一种来实现实验目标，这使得生物检测既耗时又昂贵。为了解决这一问题，我们开发了一种基于光学傅里叶变换的一台仪器具有多种功能的显微系统，其中包括用于检测的透镜系统，同时注重对生物学的适用性和易用性。该仪器可以在光路的傅里叶平面上利用微镜阵列任意调制光瞳函数进行检测。我们将本仪器命名为DIMPS(独特的光学调制光瞳函数系统)。DIMPS与传统的荧光探头和照明设备兼容，为我们提供了傅里叶滤波图像、伪浮雕图像和深度聚焦深度。此外，DIMPS通过将两个光瞳函数独立调制的图像相减，实现了110 nm的分辨率增强(伪超分辨率)。最大空间分辨率和时间分辨率分别提高到120 nm和2ms。由于DIMPS是基于继电光学的，它可以很容易地与另一种显微仪器，如共焦显微镜相结合，并提供了一种多色伪超分辨的方法。因此，DIMPS作为一种灵活的光学显微镜技术在生物研究领域显示出巨大的前景。
Optical microscopy is one of the most contributive tools for cell biology in the past decades. Many microscopic techniques with various functions have been developed to date, i.e., phase contrast microscopy, differential interference contrast (DIC) microscopy, confocal microscopy, two photon microscopy, superresolution microscopy, etc. However, person who is in charge of an experiment has to select one of the several microscopic techniques to achieve an experimental goal, which makes the biological assay time-consuming and expensive. To solve this problem, we have developed a microscopic system with various functions in one instrument based on the optical Fourier transformation with a lens system for detection while focusing on applicability and user-friendliness for biology. The present instrument can arbitrarily modulate the pupil function with a micro mirror array on the Fourier plane of the optical pathway for detection. We named the present instrument DiMPS (Distinct optical Modulated Pupil function System). The DiMPS is compatible with conventional fluorescent probes and illumination equipment, and gives us a Fourier-filtered image, a pseudo-relief image, and a deep focus depth. Furthermore, DiMPS achieved a resolution enhancement (pseudo-superresolution) of 110 nm through the subtraction of two images whose pupil functions are independently modulated. In maximum, the spatial and temporal resolution was improved to 120 nm and 2 ms, respectively. Since the DiMPS is based on relay optics, it can be easily combined with another microscopic instrument such as confocal microscope, and provides a method for multi-color pseudo-superresolution. Thus, the DiMPS shows great promise as a flexible optical microscopy technique in biological research fields.
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DOI：
10.1083/jcb.200405094
发表时间：
2005-01-03
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mimori-Kiyosue Y;Grigoriev I;Lansbergen G;Sasaki H;Matsui C;Severin F;Galjart N;Grosveld F;Vorobjev I;Tsukita S;Akhmanova A
通讯作者：
Akhmanova A
影响因子：
8.6
作者：
Fujita, Katsumasa;Kobayashi, Minoru;Kawata, Satoshi
通讯作者：
Kawata, Satoshi
DOI：
10.1073/pnas.0907866106
发表时间：
2009-12-29
影响因子：
11.1
作者：
Dertinger, T.;Colyer, R.;Enderlein, J.
通讯作者：
Enderlein, J.
影响因子：
3.3
作者：
Mizushima, N;Yamamoto, A;Ohsumi, Y
通讯作者：
Ohsumi, Y
新的人类乳腺癌细胞系KPL-4的隔离和表征，表达ERB B家族受体和白介素6。
影响因子：
8.8
作者：
Kurebayashi, J;Otsuki, T;Tang, CK;Kurosumi, M;Yamamoto, S;Tanaka, K;Mochizuki, M;Nakamura, H;Sonoo, H
通讯作者：
Sonoo, H