Identification of precursor microRNAs within distal axons of sensory neuron.
Identification of precursor microRNAs within distal axons of sensory neuron.
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DOI：
10.1111/jnc.13140
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发表时间：
2015-07
影响因子：
4.7
通讯作者：
Yoo S
中科院分区：
文献类型：
--
作者：
Kim HH;Kim P;Phay M;Yoo S
A set of specific precursor microRNAs (pre-miRNAs) are reported to localize into neuronal dendrites, where they could be processed locally to control synaptic protein synthesis and plasticity. However, it is not clear whether specific pre-miRNAs are also transported into distal axons to autonomously regulate intra-axonal protein synthesis. Here, we show that a subset of pre-miRNAs, whose mature miRNAs are enriched in axonal compartment of sympathetic neurons, are present in axons of neurons both in vivo and in vitro by quantitative PCR and by in situ hybridization. Some pre-miRNAs (let 7c-a and pre-miRs-16, 23a, 25, 125b-1, 433, and 541) showed elevated axonal levels, while others (pre-miRs-138-2, 185, and 221) were decreased in axonal levels following injury. Dicer and KSRP proteins are also present in distal axons, but Drosha is found restricted to the cell body. These findings suggest that specific pre-miRNAs are selected for localization into distal axons of sensory neurons and are presumably processed to mature miRNAs in response to extracellular stimuli. This study supports the notion that local miRNA biogenesis effectively provides another level of temporal control for local protein synthesis in axons.
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影响因子：
3
作者：
Rishal, Ida;Michaelevski, Izhak;Rozenbaum, Meir;Shinder, Vera;Medzihradszky, Katalin F.;Burlingame, Alma L.;Fainzilber, Mike
通讯作者：
Fainzilber, Mike
影响因子：
5.3
作者：
Gumy, Laura F.;Tan, Chin Lik;Fawcett, James W.
通讯作者：
Fawcett, James W.
影响因子：
4.7
作者：
Lugli, G;Larson, J;Smalheiser, NR
通讯作者：
Smalheiser, NR
影响因子：
16.2
作者：
Perry RB;Doron-Mandel E;Iavnilovitch E;Rishal I;Dagan SY;Tsoory M;Coppola G;McDonald MK;Gomes C;Geschwind DH;Twiss JL;Yaron A;Fainzilber M
通讯作者：
Fainzilber M
影响因子：
16.2
作者：
Perlson, E;Hanz, S;Fainzilber, M
通讯作者：
Fainzilber, M