Population Genomics and Haplotype Analysis in Bread Wheat Identify a Gene Regulating Glume Pubescence.
Population Genomics and Haplotype Analysis in Bread Wheat Identify a Gene Regulating Glume Pubescence.
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DOI：
10.3389/fpls.2022.897772
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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颖壳多毛是小麦的一个重要形态性状，具有较高的遗传力，与小麦对生物和非生物胁迫的抗性有关。小麦1AS染色体臂上的Hg 1（以前命名为Hg）控制颖壳多毛。Hg 1的遗传分析和定位已得到广泛研究，但仍缺乏更多有用和准确的信息，无法对Hg 1进行精细定位和候选基因的鉴定。该基因的克隆尚未报道的大型复杂的小麦基因组。在这里，我们进行了一个GWAS之间的SNP标记和颖壳短柔毛（Gp）在小麦种群352行，并进一步证明了基因表达和单倍型分析方法分离Hg 1基因。其中一个基因TraesCSU 02 G143200（TaELD 1 -1A）编码糖基转移酶样ELD 1/KOBITO 1，被鉴定为Hg 1最有希望的候选基因。通过基因注释、表达模式、功能SNP变异、单倍型分析以及花器官（穗）发育过程中的共表达分析，表明该基因可能参与颖壳短柔毛的调控。我们的研究表明，高质量的参考基因组和注释信息，以及生物信息学分析，在小麦基因克隆的重要性。
Glume hairiness or pubescence is an important morphological trait with high heritability to distinguish/characterize wheat and is related to the resistance to biotic and abiotic stresses. Hg1 (formerly named Hg) on chromosome arm 1AS controlled glume hairiness in wheat. Its genetic analysis and mapping have been widely studied, yet more useful and accurate information for fine mapping of Hg1 and identification of its candidate gene is lacking. The cloning of this gene has not yet been reported for the large complex wheat genome. Here, we performed a GWAS between SNP markers and glume pubescence (Gp) in a wheat population with 352 lines and further demonstrated the gene expression and haplotype analysis approach for isolating the Hg1 gene. One gene, TraesCSU02G143200 (TaELD1-1A), encoding glycosyltransferase-like ELD1/KOBITO 1, was identified as the most promising candidate gene of Hg1. The gene annotation, expression pattern, function SNP variation, haplotype analysis, and co-expression analysis in floral organ (spike) development indicated that it is likely to be involved in the regulation of glume pubescence. Our study demonstrates the importance of high-quality reference genomes and annotation information, as well as bioinformatics analysis, for gene cloning in wheat.
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影响因子：
12.3
作者：
Alaux M;Rogers J;Letellier T;Flores R;Alfama F;Pommier C;Mohellibi N;Durand S;Kimmel E;Michotey C;Guerche C;Loaec M;Lainé M;Steinbach D;Choulet F;Rimbert H;Leroy P;Guilhot N;Salse J;Feuillet C;International Wheat Genome Sequencing Consortium;Paux E;Eversole K;Adam-Blondon AF;Quesneville H
通讯作者：
Quesneville H
影响因子：
2
作者：
Hailu, Faris;Johansson, Eva;Merker, Arnulf
通讯作者：
Merker, Arnulf
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
2
作者：
Biffen, RH
通讯作者：
Biffen, RH
影响因子：
56.9
作者：
Avni, Raz;Nave, Moran;Distelfeld, Assaf
通讯作者：
Distelfeld, Assaf