Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray.
Urinary fetuin-A is a novel marker for diabetic nephropathy in type 2 diabetes identified by lectin microarray.
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DOI：
10.1371/journal.pone.0077118
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Makino H
中科院分区：
文献类型：
--
作者：
Inoue K;Wada J;Eguchi J;Nakatsuka A;Teshigawara S;Murakami K;Ogawa D;Terami T;Katayama A;Tone A;Iseda I;Hida K;Yamada M;Ogawa T;Makino H
我们通过凝集素微阵列分析了2型糖尿病患者在糖尿病肾病不同阶段的尿液样本，以确定预测糖尿病肾病进展的生物标志物。研究对象为日本2型糖尿病肾病各期患者，我们进行了凝集素微阵列分析（n = 17），并测定了胎球蛋白A的尿排泄量（n = 85）。 尿Siaα2- 6 Gal/GalNAc结合凝集素（SNA、SSA、TJA-I）在糖尿病肾病的发生发展过程中信号增强。接下来，我们通过使用SSA-凝集素亲和层析分离唾液酸化糖蛋白，并通过液相色谱-串联质谱仪鉴定胎球蛋白-A。在白蛋白尿（A1，0.40±0.43; A2，0.60±0.53; A3，1.57±1.13 ng/gCr; p = 7.29×10−8）和GFR分期（G1，0.39±0.39; G2，0.49±0.45; G3，1.25±1.18; G4，1.34±0.80 ng/gCr; p = 3.89×10−4）进展期间，尿中胎球蛋白A的排泄显著增加。 采用多变量logistic回归分析评估胎球蛋白A作为糖尿病肾病伴微量白蛋白尿或GFR<60 mL/min的风险。胎球蛋白A被证明是糖尿病肾病伴微量白蛋白尿和GFR降低的风险因素，比值比分别为4.721（1.881-11.844）和3.739（1.785-7.841）。总的来说，聚糖谱分析是鉴定尿生物标志物的有用方法，胎球蛋白-A是预测糖尿病肾病进展的候选物。
We analyzed the urine samples of patients with type 2 diabetes at various stages of diabetic nephropathy by lectin microarray to identify a biomarker to predict the progression of diabetic nephropathy. Japanese patients with type 2 diabetes at various stages of nephropathy were enrolled and we performed lectin microarray analyses (n = 17) and measured urinary excretion of fetuin-A (n = 85). The increased signals of urine samples were observed in Siaα2-6Gal/GalNAc-binding lectins (SNA, SSA, TJA-I) during the progression of diabetic nephropathy. We next isolated sialylated glycoproteins by using SSA-lectin affinity chromatography and identified fetuin-A by liquid chromatography–tandem mass spectrometer. Urinary excretion of fetuin-A significantly increased during the progression of albuminuria (A1, 0.40±0.43; A2, 0.60±0.53; A3 1.57±1.13 ng/gCr; p = 7.29×10−8) and of GFR stages (G1, 0.39±0.39; G2, 0.49±0.45; G3, 1.25±1.18; G4, 1.34±0.80 ng/gCr; p = 3.89×10−4). Multivariate logistic regression analysis was employed to assess fetuin-A as a risk for diabetic nephropathy with microalbuminuria or GFR<60 mL/min. Fetuin-A is demonstrated as a risk factor for both microalbuminuria and reduction of GFR in diabetic nephropathy with the odds ratio of 4.721 (1.881–11.844) and 3.739 (1.785–7.841), respectively. Collectively, the glycan profiling analysis is useful method to identify the urine biomarkers and fetuin-A is a candidate to predict the progression of diabetic nephropathy.
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影响因子：
9.8
作者：
Emoto, Masanori;Mori, Katsuhito;Nishizawa, Yoshiki
通讯作者：
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