Whole cell-SELEX aptamers for highly specific fluorescence molecular imaging of carcinomas in vivo.
Whole cell-SELEX aptamers for highly specific fluorescence molecular imaging of carcinomas in vivo.
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DOI：
10.1371/journal.pone.0070476
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Tang J
中科院分区：
文献类型：
--
作者：
Shi H;Cui W;He X;Guo Q;Wang K;Ye X;Tang J
癌症占癌症的大多数。其准确、特异的诊断对提高患者的治愈率具有重要意义。在本文中，我们报告了一个有效的例子，在体内肿瘤荧光分子成像具有极高的特异性的基础上全细胞-SELEX适配体。首先，采用抗A549肺癌细胞的核酸适体S6，用Cy 5标记作为分子成像探针。流式细胞仪检测结果表明，Cy 5-S6不仅能特异性标记体外培养的A549细胞，而且能成功地检测血清中的靶细胞。将Cy 5-S6应用于活体成像，通过系统的比较研究，证明Cy 5-S6在识别A549癌方面具有高度的特异性。特别地，将Cy 5-S6静脉注射到同时移植有A549肺癌和Tca 8113舌癌的裸鼠体内后，观察到Cy 5-S6在A549肿瘤中的更长的保留时间，并且呈现出明确的靶向癌症成像结果。在此基础上，为了进一步促进其在其他肿瘤成像中的应用，本课题组选择了两种分别针对Bel-7404和SMMC-7721肝癌细胞的适体LS 2和ZY 8，以类似的方式进行了体外和体内测试。结果表明，这些适体甚至可以有效区分同一体内不同亚型的肝癌。本研究为全细胞SELEX适体在肿瘤相关研究中的应用提供了新的思路。
Carcinomas make up the majority of cancers. Their accurate and specific diagnoses are of great significance for the improvement of patients' curability. In this paper, we report an effectual example of the in vivo fluorescence molecular imaging of carcinomas with extremely high specificity based on whole cell-SELEX aptamers. Firstly, S6, an aptamer against A549 lung carcinoma cells, was adopted and labeled with Cy5 to serve as a molecular imaging probe. Flow cytometry assays revealed that Cy5-S6 could not only specifically label in vitro cultured A549 cells in buffer, but also successfully achieve the detection of ex vivo cultured target cells in serum. When applied to in vivo imaging, Cy5-S6 was demonstrated to possess high specificity in identifying A549 carcinoma through a systematic comparison investigation. Particularly, after Cy5-S6 was intravenously injected into nude mice which were simultaneously grafted with A549 lung carcinoma and Tca8113 tongue carcinoma, a much longer retention time of Cy5-S6 in A549 tumor was observed and a clear targeted cancer imaging result was presented. On this basis, to further promote the application to imaging other carcinomas, LS2 and ZY8, which are two aptamers selected by our group against Bel-7404 and SMMC-7721 liver carcinoma cells respectively, were tested in a similar way, both in vitro and in vivo. Results showed that these aptamers were even effective in differentiating liver carcinomas of different subtypes in the same body. This work might greatly advance the application of whole cell-SELEX aptamers to carcinomas-related in vivo researches.
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影响因子：
3.8
作者：
Berman JJ
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1016197108
发表时间：
2011-03-08
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