Enterococcus faecalis reconfigures its transcriptional regulatory network activation at different copper levels.
Enterococcus faecalis reconfigures its transcriptional regulatory network activation at different copper levels.
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DOI：
10.1039/c3mt00288h
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发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
González M
中科院分区：
文献类型：
--
作者：
Latorre M;Galloway-Peña J;Roh JH;Budinich M;Reyes-Jara A;Murray BE;Maass A;González M
为了了解粪肠球菌在不同铜浓度下如何激活其表达，我们在粪肠球菌中构建了一个基因调控网络。该网络的拓扑评估显示了在其他生物中描述的常见模式。结合微阵列实验可以识别在低(0.05 mM CuSO4)和高(0.5 mM CuSO4)铜浓度下激活的子网络。分析表明铜诱导的特定功能激活模块的存在，突出了调节子LysR、ArgR为全局调节子，Copy、Fur和Lexa为局部调节子。利用粪肠球菌提供的一个孤立的体内平衡模块这一事实，我们产生了一种体内干预措施，在不影响全球转录网络的连通性的情况下将该系统从细胞中移除。这一策略使我们发现，这种细菌可以重新配置其基因表达，以维持细胞动态平衡，激活主要与葡萄糖代谢和转录过程有关的新模块。最后，这些结果将粪肠球菌定位为迄今为止拥有最完整和最可控的铜稳态系统模型的有机体。
A gene regulatory network was generated in the bacterium Enterococcus faecalis in order to understand how this organism can activate its expression under different copper concentrations. The topological evaluation of the network showed common patterns described in other organisms. Integrating microarray experiments allowed the identification of sub-networks activated under low (0.05 mM CuSO4) and high (0.5 mM CuSO4) copper concentrations. The analysis indicates the presence of specific functionally activated modules induced by copper, highlighting the regulons LysR, ArgR as global regulators and CopY, Fur and LexA as local regulators. Taking advantage of the fact that E. faecalis presented a homeostatic module isolated, we produced an in vivo intervention removing this system from the cell without affecting the connectivity of the global transcriptional network. This strategy led us to find that this bacterium can reconfigure its gene expression to maintain cellular homeostasis, activating new modules principally related to glucose metabolism and transcriptional processes. Finally, these results position E. faecalis as the organism having the most complete and controllable systemic model of copper homeostasis available to date.
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