Impact of manganese, copper and zinc ions on the transcriptome of the nosocomial pathogen Enterococcus faecalis V583.
Impact of manganese, copper and zinc ions on the transcriptome of the nosocomial pathogen Enterococcus faecalis V583.
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DOI：
10.1371/journal.pone.0026519
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kok J
中科院分区：
文献类型：
--
作者：
Abrantes MC;Lopes Mde F;Kok J
肠球菌能够应对不同环境压力的机制及其对这种生物体从共生性向致病性转变的贡献仍然知之甚少。维持金属离子的细胞内稳态对于这些细菌的生存至关重要。特别是Zn2+、Mn2+和Cu2+是非常重要的金属离子，因为它们是许多酶的辅因子，参与氧化应激防御并在宿主的免疫系统中发挥作用。它们在人体内的浓度差异很大，这使得肠球菌必须微调金属离子稳态才能在宿主体内生存并定殖。关于粪肠球菌中的金属调节知之甚少。在这里，我们首次对在高浓度锌、锰或铜离子存在下生长的粪肠球菌 V583 的基因表达进行全基因组描述。 DNA微阵列实验表明，大多数转运蛋白参与了粪肠球菌对长期暴露于高金属浓度的反应，尽管参与细胞过程、能量和氨基酸代谢的基因以及与细胞包膜相关的基因似乎也发挥着重要作用。
Mechanisms that enable Enterococcus to cope with different environmental stresses and their contribution to the switch from commensalism to pathogenicity of this organism are still poorly understood. Maintenance of intracellular homeostasis of metal ions is crucial for survival of these bacteria. In particular Zn2+, Mn2+ and Cu2+ are very important metal ions as they are co-factors of many enzymes, are involved in oxidative stress defense and have a role in the immune system of the host. Their concentrations inside the human body vary hugely, which makes it imperative for Enterococcus to fine-tune metal ion homeostasis in order to survive inside the host and colonize it. Little is known about metal regulation in Enterococcus faecalis. Here we present the first genome-wide description of gene expression of E. faecalis V583 growing in the presence of high concentrations of zinc, manganese or copper ions. The DNA microarray experiments revealed that mostly transporters are involved in the responses of E. faecalis to prolonged exposure to high metal concentrations although genes involved in cellular processes, in energy and amino acid metabolisms and genes related to the cell envelope also seem to play important roles.
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