Fusobacterium nucleatum-Induced Impairment of Autophagic Flux Enhances the Expression of Proinflammatory Cytokines via ROS in Caco-2 Cells.
Fusobacterium nucleatum-Induced Impairment of Autophagic Flux Enhances the Expression of Proinflammatory Cytokines via ROS in Caco-2 Cells.
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具核梭杆菌诱导的自噬流损伤通过 Caco-2 细胞中的 ROS 增强促炎细胞因子的表达
DOI：
10.1371/journal.pone.0165701
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zeng DZ
中科院分区：
文献类型：
--
作者：
Tang B;Wang K;Jia YP;Zhu P;Fang Y;Zhang ZJ;Mao XH;Li Q;Zeng DZ
具核梭杆菌（Fusobacterium nucleatum, F.核仁）在胃肠炎症中起关键作用。然而，F.细胞核对炎症的作用尚不清楚。本研究表明，F. nucleatum可诱导Caco-2结肠腺癌细胞产生促炎性细胞因子IL-8、IL-1β和TNF-α及活性氧。此外，ROS清除剂（NAC或Tiron）可降低F.细胞核感染。此外，我们还观察到，在Caco-2细胞中，F.细胞核感染。结果：F.在Caco-2细胞中，用自噬药理学抑制剂（3-甲基腺嘌呤，巴弗洛霉素A1）或RNA干扰必需自噬基因（ATG 5或ATG 12）可增强细胞核。这些结果表明，F.细胞核诱导的自噬流损伤通过ROS增强了促炎细胞因子在Caco-2细胞中的表达。
Fusobacterium nucleatum (F. nucleatum) plays a critical role in gastrointestinal inflammation. However, the exact mechanism by which F. nucleatum contributes to inflammation is unclear. In the present study, it was revealed that F. nucleatum could induce the production of proinflammatory cytokines (IL-8, IL-1β and TNF-α) and reactive oxygen species (ROS) in Caco-2 colorectal) adenocarcinoma cells. Furthermore, ROS scavengers (NAC or Tiron) could decrease the production of proinflammatory cytokines during F. nucleatum infection. In addition, we observed that autophagy is impaired in Caco-2 cells after F. nucleatum infection. The production of proinflammatory cytokines and ROS induced by F. nucleatum was enhanced with either autophagy pharmacologic inhibitors (3-methyladenine, bafilomycin A1) or RNA interference in essential autophagy genes (ATG5 or ATG12) in Caco-2 cells. Taken together, these results indicate that F. nucleatum-induced impairment of autophagic flux enhances the expression of proinflammatory cytokines via ROS in Caco-2 Cells.
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影响因子：
29.4
作者：
Raju D;Hussey S;Ang M;Terebiznik MR;Sibony M;Galindo-Mata E;Gupta V;Blanke SR;Delgado A;Romero-Gallo J;Ramjeet MS;Mascarenhas H;Peek RM;Correa P;Streutker C;Hold G;Kunstmann E;Yoshimori T;Silverberg MS;Girardin SE;Philpott DJ;El Omar E;Jones NL
通讯作者：
Jones NL
影响因子：
3.7
作者：
Liu P;Liu Y;Wang J;Guo Y;Zhang Y;Xiao S
通讯作者：
Xiao S
影响因子：
13.3
作者：
Cremer TJ;Amer A;Tridandapani S;Butchar JP
通讯作者：
Butchar JP
影响因子：
--
作者：
Marcato, L. G.;Ferlini, A. P.;Sobrinho, A. P. R.
通讯作者：
Sobrinho, A. P. R.
影响因子：
5.8
作者：
Kayath, Christian Aime;Hussey, Seamus;Allaoui, Abdelmounaaim
通讯作者：
Allaoui, Abdelmounaaim