Eavesdropping and crosstalk between secreted quorum sensing peptide signals that regulate bacteriocin production in Streptococcus pneumoniae.
Eavesdropping and crosstalk between secreted quorum sensing peptide signals that regulate bacteriocin production in Streptococcus pneumoniae.
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DOI：
10.1038/s41396-018-0178-x
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Rozen DE
中科院分区：
文献类型：
--
作者：
Miller EL;Kjos M;Abrudan MI;Roberts IS;Veening JW;Rozen DE
群体感应(QS)是细菌分泌化学信号并对化学信号做出反应以协调整个种群行为的地方，它揭示了细菌具有高度的社会性。在这里，我们研究了QS信号和受体的多样性如何改变QS系统控制的社会相互作用，QS系统调节肺炎链球菌细菌素的分泌，由BLP操纵子(细菌素样肽)编码。对4096株肺炎球菌基因组进行分析，发现9个BLP QS信号(BlpC)和5个QS受体群(BlpH)。信号和受体之间不完美的一致性表明细胞之间存在广泛的社会互动，特别是窃听(细胞对自己不产生的信号做出反应)和串扰(细胞产生非克隆人检测到的信号)。通过测量含有六种不同BLP QS受体的报告菌株对同源和非同源多肽的反应，在体外证实了这一点。相邻生长的肺炎球菌菌落之间的分析进一步证明，串扰和窃听发生在信号分泌的内源性水平上。最后，对产生细菌素的QS菌株的模拟表明，即使在对非同源信号的亲和力非常弱的情况下，窃听也可能对进化有益。我们的结果强调了社会相互作用可以调节细菌之间的种内竞争，并揭示了竞争相互作用可以被多态的QS系统改变。
Quorum sensing (QS), where bacteria secrete and respond to chemical signals to coordinate population-wide behaviors, has revealed that bacteria are highly social. Here, we investigate how diversity in QS signals and receptors can modify social interactions controlled by the QS system regulating bacteriocin secretion in Streptococcus pneumoniae, encoded by the blp operon (bacteriocin-like peptide). Analysis of 4 096 pneumococcal genomes detected nine blp QS signals (BlpC) and five QS receptor groups (BlpH). Imperfect concordance between signals and receptors suggested widespread social interactions between cells, specifically eavesdropping (where cells respond to signals that they do not produce) and crosstalk (where cells produce signals that non-clones detect). This was confirmed in vitro by measuring the response of reporter strains containing six different blp QS receptors to cognate and non-cognate peptides. Assays between pneumococcal colonies grown adjacent to one another provided further evidence that crosstalk and eavesdropping occur at endogenous levels of signal secretion. Finally, simulations of QS strains producing bacteriocins revealed that eavesdropping can be evolutionarily beneficial even when the affinity for non-cognate signals is very weak. Our results highlight that social interactions can mediate intraspecific competition among bacteria and reveal that competitive interactions can be modified by polymorphic QS systems.
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影响因子：
9.4
作者：
McGee, L;McDougal, L;Klugman, KP
通讯作者：
Klugman, KP
影响因子：
3.1
作者：
Dawid, Suzanne;Roche, Aoife M.;Weiser, Jeffrey N.
通讯作者：
Weiser, Jeffrey N.
DOI：
10.1073/pnas.1102923108
发表时间：
2011-08-16
影响因子：
11.1
作者：
Eldar, Avigdor
通讯作者：
Eldar, Avigdor
影响因子：
5.2
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Ng JC;Chan Y;Tun HM;Leung FC;Shin PK;Chiu JM
通讯作者：
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影响因子：
3.3
作者：
Miller EL;Evans BA;Cornejo OE;Roberts IS;Rozen DE
通讯作者：
Rozen DE