Targeting mesenchymal stem cells to activated endothelial cells.
Targeting mesenchymal stem cells to activated endothelial cells.
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DOI：
10.1016/j.biomaterials.2009.03.038
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发表时间：
2009-08
期刊：
影响因子：
14
通讯作者：
Dennis, James E.
中科院分区：
文献类型：
--
作者：
Ko, In Kap;Kean, Thomas J.;Dennis, James E.
细胞表面包衣是一种方法，其中特定的分子暂时锚定在细胞膜上，以调节细胞的行为。细胞表面涂层是一种通过结合细胞间细胞粘附分子-1 （ICAM-1）将间充质干细胞（MSC）递送至内皮细胞的方法。包被棕榈化蛋白G （PPG）和ICAM-1抗体（AbICAM-1）的MSCs，在ICAM-1包被的盖片上孵育，其细胞结合比仅包被PPG的对照组增加了40倍。abicam -1包被的MSCs与人血管内皮细胞（HUVECs）孵育，无论是否暴露于TNFα（上调ICAM-1表达），与仅ppg对照相比，与对照HUVECs的结合增加2.6倍，与TNFα处理的HUVECs的结合增加16倍。用ICAM-1抗体预处理HUVECs促进了PPG-only MSCs的附着，同时使abicam -1 MSCs的附着减少了约50%。在tnf α刺激的HUVECs的流动室研究中，PPG-only和MSC-only在4 dynes/cm2时失去了80-90%的初始结合，而AbICAM-1-MSCs在4 dynes/cm2时保持100%的结合，在25 dynes/cm2时保持40%的结合。这些结果表明，细胞表面涂层促进了间充质干细胞与内皮细胞的附着，并为干细胞递送到炎症部位提供了一种方法。
Cell surface coating is a methodology wherein specific molecules are transiently anchored onto cell membrane to modulate cell behavior. Cell surface coating was tested as a method to deliver mesenchymal stem cells (MSC) to endothelial cells via binding to intercellular cell adhesion molecule-1 (ICAM-1). MSCs coated with palmitated protein G (PPG) followed by antibodies to ICAM-1 (AbICAM-1), and incubated on ICAM-I coated coverslips showed a 40-fold increase in cell binding over PPG-only controls. AbICAM-1-coated MSCs incubated with human vascular endothelial cells (HUVECs), with and without exposure to TNFα (to upregulate ICAM-1 expression), showed 2.6-fold increased binding to control HUVECs over PPG-only controls, and a 16-fold increase in binding to TNFα-treated HUVECs. Pretreatment of HUVECs with ICAM-1 antibody promoted the attachment of PPG-only MSCs while reducing the attachment of AbICAM-1-MSCs by approximately 50%. In flow chamber studies on TNFα-stimulated HUVECs, PPG-only, and MSC-only lost 80–90% of their initial binding at 4 dynes/cm2, while AbICAM-1-MSCs maintained 100% binding at 4 dynes/cm2 and 40% binding at 25 dynes/cm2. These results demonstrate that cell surface coating promotes the attachment of MSCs to endothelial cells, and provides a methodology for the delivery of stem cells to sites of inflammation.
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炎症引起的白细胞跨淋巴管内皮迁移的机制。
影响因子：
15.3
作者：
Johnson, Louise A;Clasper, Steven;Holt, Andrew P;Lalor, Patricia F;Baban, Dilair;Jackson, David G
通讯作者：
Jackson, David G
影响因子：
2.8
作者：
Dennis, JE;Cohen, N;Caplan, AI
通讯作者：
Caplan, AI
DOI：
10.1073/pnas.92.11.4857
发表时间：
1995-05-23
影响因子：
11.1
作者：
PEREIRA, RF;HALFORD, KW;PROCKOP, DJ
通讯作者：
PROCKOP, DJ
影响因子：
4.8
作者：
Maitra, B;Szekely, E;Koç, O
通讯作者：
Koç, O
影响因子：
5.1
作者：
Darling, D;GaleaLauri, J;Farzaneh, F
通讯作者：
Farzaneh, F