Applications of a novel biodetection system to saliva using protein fingerprints with data processing.
Applications of a novel biodetection system to saliva using protein fingerprints with data processing.
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使用蛋白质指纹和数据处理将新型生物检测系统应用于唾液。
DOI：
10.1016/j.bmc.2018.04.049
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Nokihara K
中科院分区：
文献类型：
--
作者：
Tominaga Y;Usui K;Hirata A;Ito H-O;Nokihara K
一个基本的方法已经开发出专注于牙周病的简便和快速的检查。牙周病是一种口腔疾病，据认为影响80%的成年人，早期发现和治疗是改善生活质量的理想选择。不幸的是，传统的方法并不一致，因为这种疾病是由一些不确定的细菌引起的，检测依赖于牙医的技能。因此，需要客观的检测系统。我们已经使用一种新的生物检测系统，命名为PepTenChip®，对唾液进行了实验。使用来自健康受试者的样本和血红蛋白（f-Hb）和乳酸脱氢酶（LDH）的混合物制备唾液的疾病模型，所述乳酸脱氢酶（LDH）用作健康唾液中的牙周疾病标志蛋白。PepTenChip®是一种肽微阵列，其中荧光标记的结构化肽固定在新型无定形碳基质上。由于用作捕获分子的肽是荧光标记的，因此不需要标记分析物。检测应用分析物前后的荧光强度变化，而不是使用一组抗原-抗体的常规微阵列常见的ON/OFF检测。荧光强度值根据捕获的蛋白质的浓度而变化，从而允许产生蛋白质指纹（PFP）和树状图。与常规生物检测不同，本方法不依赖于“一对一”相互作用，并且在疾病原因不明确或未知的情况下可以设想优点。统计分析，如多变量分析，允许将添加到唾液中的蛋白质类型分类为疾病模拟物。PepTenChip®系统是有用的和方便的牙周疾病的检查在医疗保健。
A fundamental method has been developed focusing on a facile and rapid examination of periodontal disease. Periodontal disease is an oral disease thought to affect 80% of adults, and early detection with treatment is desirable for the improvement of the quality of life. Unfortunately conventional methods are not consistent as the disease is caused by a number of undefined bacteria and detection relies on the skills of the dentist. Thus an objective detection system is required. We have performed an experiment on saliva using a novel biodetection system, designated PepTenChip®. A disease model for saliva was prepared using a specimen from a healthy subject and a mixture of hemoglobin (f-Hb) and lactate dehydrogenase (LDH), which is used as a periodontal disease marker protein with healthy saliva. PepTenChip® is a peptide microarray in which fluorescent labelled structured peptides are immobilized on a novel amorphous carbon substrate. Since the peptides used as capture molecules are fluorescently labelled, labeling of analytes is not necessary. The fluorescence intensity change before and after application of analytes are detected rather than the ON/OFF detection common to conventional microarrays using a set of antigen–antibody. The fluorescence intensity value changes according to the concentration of captured protein allowing the generation of protein fingerprint (PFP) and dendrograms. The present method does not rely on a “one to one” interaction, unlike conventional biodetection, and advantages can be envisaged in the case of an undefined or unknown cause of disease. The statistical analyses, such as multivariate analyses, allow classification of the type of proteins added in saliva as mimetics of disease. PepTenChip® system is useful and convenient for examination of periodontal disease in health care.
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使用设计的合成肽阵列开发实用的蛋白质芯片
影响因子：
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作者：
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作者：
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使用荧光标记的 PrP 肽区分 L 型非典型和经典 BSE 以及痒病的新型检测方法
影响因子：
3.5
作者：
Kazuo Kasai;A. Hirata;T. Ohyama;K. Nokihara;T. Yokoyama;S. Mohri
通讯作者：
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影响因子：
--
作者：
Usui, K;Tomizaki, K;Mihara, H
通讯作者：
Mihara, H