Parathyroid hormone-related protein in the cardiovascular system.
Parathyroid hormone-related protein in the cardiovascular system.
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心血管系统中的甲状旁腺激素相关蛋白。
DOI：
10.1210/endo.135.1.8013360
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发表时间：
1994
期刊：
影响因子：
4.8
通讯作者：
L. Deftos
中科院分区：
文献类型：
--
作者：
D. Burton;D. W. Brandt;L. Deftos
PTH相关蛋白（PTHrP）在几种类型的平滑肌中以牵张反应的方式表达。我们之前证明了成年大鼠心肌中PTHrP的产生。在这项研究中，我们证明了PTHrP的生产在几种哺乳动物的心血管系统，包括人类。我们证明PTHrP的免疫组织学，使用一组鼠PTHrP表位的单克隆抗体，跨越整个长度的人PTHrP氨基酸序列，定量的PTHrP在大鼠心血管系统的浓度区域特异性RIA，并测量PTHrP信使RNA（mRNA）的相对水平的竞争性聚合酶链反应。免疫组织学研究表明PTHrP存在于人、大鼠、猪和兔的心血管系统中。最强的表达被发现在心房，其次是大血管，然后是心室。心脏左右两侧未见差异。双染显示PTHrP和心房肽在部分细胞中共表达。使用RIA和聚合酶链反应，我们表明，心房含有较高浓度的PTHrP比心室和PTHrP的相对浓度相关的mRNA浓度在这两个组织。主动脉和腔静脉周围平滑肌中PTHrP的浓度与心房中的浓度相当。然而，在这些大血管中，较高的PTHrP水平并不对应于其mRNA水平。而PTHrP的免疫反应浓度在心房，主动脉和腔静脉是相似的，在主动脉和腔静脉的mRNA水平比心房低3倍。某些PTHrP表位似乎在特定的心血管组织中差异表达。区域特异性测定的比较显示，通过免疫测定法测量的对PTHrP-（38 - 64）和PTHrP-（109 - 141）的免疫反应性比通过免疫测定法测定的对PTHrP-（1 - 34）的免疫反应性高3 - 5倍。我们的观察结果表明，心房，主动脉和腔静脉含有最大量的PTHrP在心血管系统。主动脉和腔静脉中PTHrP及其mRNA的浓度之间的差异表明，这两个可能是在这些组织中不同的调节。PTHrP的广泛分布表明该蛋白在心血管系统中具有重要功能，可能作为心房钠尿肽钠调节轴的钙对应物发挥作用。
PTH-related protein (PTHrP) is expressed in a stretch-responsive manner in several types of smooth muscle. We previously demonstrated the production of PTHrP in adult rat heart muscle. In this study, we demonstrate the production of PTHrP in the cardiovascular systems of several mammalian species, including human. We demonstrate PTHrP by immunohistology, using a panel of murine monoclonal antibodies to PTHrP epitopes that span the entire length of the human PTHrP amino acid sequence, quantitate the concentration of PTHrP in the rat cardiovascular system by region-specific RIAs, and measure the relative levels of PTHrP messenger RNA (mRNA) by competitive polymerase chain reaction. Immunohistology studies demonstrated the presence of PTHrP in the cardiovascular systems of humans, rats, pigs, and rabbits. The most robust expression was found in atria, followed by the large vessels, then ventricles. No difference was seen between the left and right sides of the heart. Double staining procedures revealed that PTHrP and atrial naturietic peptide were coexpressed in some cells. Using RIAs and polymerase chain reaction, we demonstrated that atria contained a higher concentration of PTHrP than ventricles and that the relative PTHrP concentrations correlated to its mRNA concentrations in these two tissues. The concentrations of PTHrP in the smooth muscle surrounding the aorta and vena cava were comparable to those in atria. However, in these large vessels, the higher PTHrP levels did not correspond to its mRNA levels. Whereas the immunoreactive concentrations of PTHrP were similar in the atria, aorta, and vena cava, the mRNA levels in the aorta and vena cava were 3-fold lower than those in the atria. Certain PTHrP epitopes appeared to be differentially expressed in specific cardiovascular tissues. A comparison of region-specific assays showed that immunoreactivity measured by immunoassays to PTHrP-(38-64) and PTHrP-(109-141) were 3- to 5-fold greater than that determined by an immunoassay to PTHrP-(1-34). Our observations demonstrate that the atria, aorta, and vena cava contain the greatest amounts of PTHrP in the cardiovascular system. The discrepancy between the concentrations of PTHrP and its mRNA present in the aorta and vena cava suggest that the two may be regulated differently in these tissues. The widespread distribution of PTHrP suggests an important function for the protein in the cardiovascular system, possibly functioning as the calcium counterpart for the atrial natriuretic-sodium regulatory axis.
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影响因子：
10.5
作者：
G. Strewler;R. Nissenson
通讯作者：
G. Strewler;R. Nissenson
DOI：
10.1073/pnas.87.18.6969
发表时间：
1990-09
影响因子：
11.1
作者：
M. A. Thiede;A G Daifotis;E. C. Weir;M L Brines;W. Burtis;K. Ikeda;B. E. Dreyer;R E Garfield;A. Broadus
通讯作者：
M. A. Thiede;A G Daifotis;E. C. Weir;M L Brines;W. Burtis;K. Ikeda;B. E. Dreyer;R E Garfield;A. Broadus
甲状旁腺激素相关蛋白：结构、功能和测量。
影响因子：
9.3
作者：
Burtis,WJ
通讯作者：
Burtis,WJ
影响因子：
11.2
作者：
R. Campos;S. Asa;D. Drucker
通讯作者：
R. Campos;S. Asa;D. Drucker
甲状旁腺激素及甲状旁腺激素相关蛋白与兔肾微血管血管平滑肌的结合。
影响因子：
4.8
作者：
Nickols,GA;Nickols,MA;Helwig,JJ
通讯作者：
Helwig,JJ