Primordial germ cells originate from the endodermal strand cells in the ascidian Ciona intestinalis
Primordial germ cells originate from the endodermal strand cells in the ascidian Ciona intestinalis
复制标题
原始生殖细胞起源于海鞘的内胚层链细胞
DOI：
10.1007/s00427-001-0204-1
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发表时间：
2002
影响因子：
2.4
通讯作者：
Y. Yamaguchi
中科院分区：
文献类型：
--
作者：
K. Takamura;M. Fujimura;Y. Yamaguchi
抽象的。海鞘内生殖细胞的来源仍不清楚。在此之前，我们通过聚合酶链式反应从卵巢组织的cDNA文库中克隆了一条肠黄瓜的VasA同源物(CiVH)，并证明其在成年和幼年期的生殖细胞中具有特异性表达。在本研究中，我们制备了一种抗CiVH蛋白的单抗，并追踪了该抗体从尾芽中期到成年的染色过程。结果表明，尾芽中期胚胎和幼虫内胚链中均有阳性细胞。当幼虫的尾巴在变态过程中被吸收到躯干时，CiVH阳性细胞从尾部的碎片迁移到发育中的性腺雏形中，并在幼虫体内形成原始生殖细胞(PGC)。精巢雏形与性腺雏形分离，其馀部分分化为卵巢。精巢原基和卵巢原基的原生殖细胞分别分化为生精细胞和卵母细胞。去掉含有抗体阳性细胞的幼虫尾巴，变态后的仔鱼不含任何CiVH阳性细胞，表明幼虫体内的PGCs来自幼虫尾部的一部分。然而，即使在这样的幼体中，在性腺雏形的后期也出现了新的阳性细胞。这一观察结果表明，一种代偿机制调节了肠线虫生殖系的形成。
Abstract. The origin of germ cells in the ascidian is still unknown. Previously, we cloned a vasa homologue (CiVH) of Ciona intestinalis from the cDNA library of ovarian tissue by polymerase chain reaction and showed that its expression was specific to germ cells in adult and juvenile gonads. In the present study, we prepared a monoclonal antibody against CiVH protein and traced the staining for this antibody from the middle tailbud stage to young adulthood. Results showed that positive cells are present in the endodermal strand in middle tailbud embryos and larvae. When the larval tail was absorbed into the trunk during metamorphosis, the CiVH-positive cells migrated from the debris of the tail into the developing gonad rudiment, and appeared to give rise to a primordial germ cell (PGC) in the young juvenile. The testis rudiment separated from the gonad rudiment, the remainder of which differentiated into the ovary. PGCs of the testis rudiment and the ovary rudiment differentiated into spermatogenic and oogenic cells, respectively. When the larval tail containing the antibody-positive cells was removed, the juveniles did not contain any CiVH-positive cells after metamorphosis, indicating that the PGCs in the juvenile originated from part of the larval tail. However, even in such juveniles, positive cells newly appeared in the gonad rudiment at a later stage. This observation suggests that a compensatory mechanism regulates germline formation in C. intestinalis.
DOI：
10.1073/pnas.93.24.13837
发表时间：
1996-11-26
影响因子：
11.1
作者：
Gruidl, ME;Smith, PA;Bennett, KL
通讯作者：
Bennett, KL
海鞘追逐尾巴：对脊索动物起源和进化的发育洞察。
DOI：
10.1016/s0168-9525(00)89106-4
发表时间：
1995
期刊：
Trends in genetics : TIG
影响因子：
--
作者：
Satoh,N;Jeffery,WR
通讯作者：
Jeffery,WR