Fabrication and Characterization of Electrospun Thermoplastic Polyurethane/Fibroin Small-Diameter Vascular Grafts for Vascular Tissue Engineering.
Fabrication and Characterization of Electrospun Thermoplastic Polyurethane/Fibroin Small-Diameter Vascular Grafts for Vascular Tissue Engineering.
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DOI：
10.3139/217.3247
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发表时间：
2016-11
期刊：
影响因子：
--
通讯作者：
Turng LS
中科院分区：
文献类型：
--
作者：
Yu E;Zhang J;Thomson JA;Turng LS
由于自体移植血管的短缺以及合成移植物的血栓形成和内膜增生问题，对小直径血管替代品的需求一直在增加。在这项研究中，由热塑性聚氨酯（TPU）和丝素蛋白制成的混合小直径血管移植物，具有排列内层和随机外层的混合纤维结构，通过静电纺丝技术使用定制的条纹收集器同时产生排列和随机纤维来制造。甲醇后处理过程诱导丝心蛋白构象从水溶性、无定形和不太有序的结构转变为不溶于水的β-折叠结构，该结构具有强大的机械性能和相对缓慢的蛋白水解降解。甲醇后处理还产生了模仿天然血管中胶原纤维的波浪结构的卷曲纤维。在电纺TPU/丝心蛋白样品上发现超细纳米纤维和纳米网，有效增加了细胞粘附和迁移的表面积。循环圆周拉伸测试结果显示，与人类冠状动脉相比，由软 TPU/丝心蛋白混合物制成的移植物具有相容的机械性能。此外，培养6天和60天后的内皮细胞细胞培养测试显示出TPU/丝素蛋白移植物的高细胞活力和良好的生物相容性，表明电纺TPU/丝素蛋白移植物在血管组织工程中的应用潜力。
The demand for small-diameter blood vessel substitutes has been increasing due to a shortage of autograft vessels and problems with thrombosis and intimal hyperplasia with synthetic grafts. In this study, hybrid small-diameter vascular grafts made of thermoplastic polyurethane (TPU) and silk fibroin, which possessed a hybrid fibrous structure of an aligned inner layer and a random outer layer, were fabricated by the electrospinning technique using a customized striated collector that generated both aligned and random fibers simultaneously. A methanol post-treatment process induced the transition of fibroin protein conformation from the water-soluble, amorphous, and less ordered structures to the water-insoluble β-sheet structures that possessed robust mechanical properties and relatively slow proteolytic degradation. The methanol post-treatment also created crimped fibers that mimicked the wavy structure of collagen fibers in natural blood vessels. Ultrafine nanofibers and nanowebs were found on the electrospun TPU/fibroin samples, which effectively increased the surface area for cell adhesion and migration. Cyclic circumferential tensile test results showed compatible mechanical properties for grafts made of a soft TPU/fibroin blend compared to human coronary arteries. In addition, cell culture tests with endothelial cells after 6 and 60 days of culture exhibited high cell viability and good biocompatibility of TPU/fibroin grafts, suggesting the potential of applying electrospun TPU/fibroin grafts in vascular tissue engineering.
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