Alz‐50 immunoreactivity in the hypothalamus of the normal and Alzheimer human and the rat
Alz‐50 immunoreactivity in the hypothalamus of the normal and Alzheimer human and the rat
复制标题
正常人和阿尔茨海默病人和大鼠下丘脑中的 Alz-50 免疫反应性
DOI：
10.1002/cne.903060406
复制
发表时间：
1991
影响因子：
2.5
通讯作者：
P. Davies
中科院分区：
文献类型：
--
作者：
W. Byne;L. Mattiace;Y. Kress;P. Davies
Alz-50是一种单克隆抗体，可识别阿尔茨海默病（AD）中丰富的68千道尔顿蛋白质，但在正常大脑中无法通过免疫印迹方法检测到。当用于AD皮质的免疫组织化学时，Alz-50识别大量神经元缠结（NFT）、神经炎斑块和一些通过常规组织病理学染色方法未显示神经元变性证据的神经元，在正常和AD受试者尸检时获得的脑下丘脑中的光镜和电镜水平描述了Alz-50免疫反应性。还描述了大鼠下丘脑中的Alz-50免疫反应性。在正常人和大鼠中均鉴定出Alz-50免疫反应性下丘脑神经元的明确群体。在正常人的光镜水平，免疫反应神经元主要集中在室周区，但也分散在整个弓状核（ARC），下丘脑外侧区，结节区。免疫反应阳性纤维见于室周区、腹内侧核背侧部（VMNd）、ARC和正中隆起外层（ME）。在大鼠中，反应性神经元只出现在室周区，反应性纤维出现在室周区，内侧视前核复合体，视交叉上核，VMNd，ARC，和外层的ME。超微结构，所有的免疫反应在正常人和大鼠下丘脑与神经元内囊泡。在AD下丘脑中，除了正常大脑中染色的细胞和纤维外，Alz-50还鉴定出许多老年斑和NFT。免疫反应性斑块和NFT最多的地区，以前报道的经历神经退行性变。在超微结构水平，在AD下丘脑的免疫反应与丝以及囊泡。Alz-50对特定囊泡群体的选择性染色的意义尚不清楚;然而，目前的结果表明，它与AD病理学无关。
Alz‐50 is a monoclonal antibody recognizing a 68 kilodalton protein that is abundant in Alzheimer's disease (AD) but not detectable by immunoblotting methods in normal brains. When used for immunohistochemistry in AD cortex, Alz‐50 recognizes large numbers of neurofibrillary tangles (NFT), neuritic plaques, and some neurons that show no evidence of neurofibrillary degeneration by conventional histopathological staining methods, Alz‐50 immunoreactivity is described at the light and electron microscopic levels in the hypothalamus of brains obtained at autopsy from normal and AD subjects. Alz‐50 immunoreactivity in the rat hypothalamus is also described. A well‐defined population of Alz‐50 immunoreactive hypothalamic neurons was identified in both the normal human and rat. At the light microscopic level in the normal human, immunoreactive neurons were most concentrated in the periventricular region, but were also scattered throughout the arcuate nucleus (ARC), lateral hypothalamic area, and tuberal region. Immunoreactive fibers were seen in the periventricular region, dorsal division of the ventromedial nucleus (VMNd), ARC, and external layer of the median eminence (ME). In the rat, reactive neurons were seen only in the periventricular region, and reactive fibers were seen in the periventricular zone, medial preoptic nuclear complex, suprachiasmatic nucleus, VMNd, ARC, and external layer of the ME. Ultrastructurally, all immunoreactivity in the normal human and rat hypothalamus was associated with intraneuronal vesicles. In the AD hypothalamus, Alz‐50 identified numerous senile plaques and NFT in addition to the cells and fibers that were stained in the normal brains. Immunoreactive plaques and NFT were most numerous in regions previously reported to undergo neurofibrillary degeneration. At the ultrastructural level, the immunoreactivity in the AD hypothalamus was associated with filaments as well as vesicles. The significance of the selective staining of a specific population of vesicles by Alz‐50 is unknown; however, the present results suggest that it is independent of AD pathology.
影响因子：
56.9
作者：
B. Wolozin;Alex Pruchnicki;D. Dickson;P. Davies
通讯作者：
B. Wolozin;Alex Pruchnicki;D. Dickson;P. Davies
Alz 50 是一种针对阿尔茨海默病抗原的单克隆抗体，可与来自牛和正常人脑的 tau 蛋白发生交叉反应。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ksiezak-Reding,H;Davies,P;Yen,SH
通讯作者：
Yen,SH
影响因子：
--
作者：
PAPASOZOMENOS, SC;BINDER, LI
通讯作者：
BINDER, LI