B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.
B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.
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DOI：
10.1186/1742-4690-6-104
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发表时间：
2009-11-16
期刊：
影响因子：
3.3
通讯作者：
Møller-Larsen A
中科院分区：
文献类型：
--
作者：
Brudek T;Christensen T;Aagaard L;Petersen T;Hansen HJ;Møller-Larsen A
神经变性疾病多发性硬化症（MS）的病因尚不清楚。主要的假设认为，多发性硬化症是遗传易感个体暴露于某些环境因素的结果。疱疹病毒和人内源性逆转录病毒（herv）是多发性硬化症发生的潜在重要因素。疱疹病毒可以激活herv，而herv在多发性硬化症患者中被激活。利用流式细胞术，我们分析了MS活动性和稳定性患者以及对照个体PBMCs表面HERV-H Env和HERV-W Env表位的表达。我们还分析了表达的HERV-H和HERV-W Env表位的血清抗体水平。我们发现，与稳定型MS患者或对照个体相比，活动性MS患者的B细胞和单核细胞上HERV-H和HERV-W Env表位的表达明显更高。此外，活动性疾病患者在PBMC人群中具有相对较高的B细胞数量，并且对HERV-H Env和HERV-W Env表位具有较高的抗体反应性。活动性MS患者血清中较高的抗体反应性与B细胞和单核细胞中较高水平的HERV-H Env和HERV-W Env表达相关。我们没有在稳定的MS患者或对照组中发现这种相关性。这些发现表明，HERV-H Env和HERV-W Env在活动性MS患者的B细胞和单核细胞表面表达量较高，并且这些蛋白的表达可能与疾病的恶化有关。
The etiology of the neurogenerative disease multiple sclerosis (MS) is unknown. The leading hypotheses suggest that MS is the result of exposure of genetically susceptible individuals to certain environmental factor(s). Herpesviruses and human endogenous retroviruses (HERVs) represent potentially important factors in MS development. Herpesviruses can activate HERVs, and HERVs are activated in MS patients. Using flow cytometry, we have analyzed HERV-H Env and HERV-W Env epitope expression on the surface of PBMCs from MS patients with active and stable disease, and from control individuals. We have also analyzed serum antibody levels to the expressed HERV-H and HERV-W Env epitopes. We found a significantly higher expression of HERV-H and HERV-W Env epitopes on B cells and monocytes from patients with active MS compared with patients with stable MS or control individuals. Furthermore, patients with active disease had relatively higher numbers of B cells in the PBMC population, and higher antibody reactivities towards HERV-H Env and HERV-W Env epitopes. The higher antibody reactivities in sera from patients with active MS correlate with the higher levels of HERV-H Env and HERV-W Env expression on B cells and monocytes. We did not find such correlations for stable MS patients or for controls. These findings indicate that both HERV-H Env and HERV-W Env are expressed in higher quantities on the surface of B cells and monocytes in patients with active MS, and that the expression of these proteins may be associated with exacerbation of the disease.
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影响因子：
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