Identification of candidate odorant receptors in Asian corn borer Ostrinia furnacalis.
Identification of candidate odorant receptors in Asian corn borer Ostrinia furnacalis.
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DOI：
10.1371/journal.pone.0121261
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Matsuo T
中科院分区：
文献类型：
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作者：
Yang B;Ozaki K;Ishikawa Y;Matsuo T
在鳞翅目昆虫中，气味受体参与性信息素和一般气味的感知。在亚洲玉米螟中，虽然已经鉴定了几种信息素受体，但没有一般气味受体的报道。在这项研究中，进行了RNA测序分析，以确定整个剧目的气味受体表达的触角。菜在从雌雄蛾触角获得的1200万个读数中，鉴定了52个候选气味受体，其中包括45个新的气味受体。通过读码图谱和定量逆转录PCR估计候选气味受体的表达水平。这些分析证实了先前鉴定的信息素受体的表达是高度雄性偏向的。相比之下，没有一个新发现的气味受体表现出男性偏好的表达。三个新发现的气味受体表现出女性偏见的表达。其中两个是最高表达的气味受体在女性触角，这表明他们可能参与检测气味的重要诱导女性特定的行为，如产卵地点选择。此外，还鉴定了21种离子型受体、5种味觉受体、2种感觉神经元膜蛋白和26种气味降解酶的候选基因。我们的研究结果提供了进一步分析的化学感受系统在鸵鸟属物种的基础。
In lepidopteran insects, odorant receptors are involved in the perception of sex pheromones and general odorants. In the Asian corn borer, Ostrinia furnacalis, although several pheromone receptors have been identified, no general odorant receptor has been reported. In this study, an RNA sequencing analysis was carried out to identify the whole repertoire of the odorant receptors expressed in the antennae of O. furnacalis. Among 12 million reads obtained from the antennae of male and female moths, 52 candidate odorant receptors were identified, including 45 novel ones. Expression levels of candidate odorant receptors were estimated by read mapping and quantitative reverse transcription PCR. These analyses confirmed that the expression of the previously identified pheromone receptors was highly male biased. In contrast, none of the newly identified odorant receptors showed male-biased expression. Three of the newly identified odorant receptors showed female-biased expression. Two of them were the most highly expressed odorant receptors in the female antennae, suggesting that they may be involved in the detection of odorants important for the induction of female-specific behaviors such as oviposition site selection. In addition, candidate genes of 21 ionotropic receptors, 5 gustatory receptors, 2 sensory neuron membrane proteins, and 26 odorant degrading enzymes were identified. Our results provide a basis for further analysis of the chemosensory system in the Ostrinia species.
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DOI：
10.1093/bioinformatics/btq224
发表时间：
2010-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
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作者：
Katoh K;Toh H
通讯作者：
Toh H
DOI：
10.1073/pnas.1204661109
发表时间：
2012-08-28
影响因子：
11.1
作者：
Leary, Greg P.;Allen, Jean E.;Wanner, Kevin W.
通讯作者：
Wanner, Kevin W.
DOI：
10.1073/pnas.1017963108
发表时间：
2011-05-03
影响因子：
11.1
作者：
Grosse-Wilde, Ewald;Kuebler, Linda S.;Hansson, Bill S.
通讯作者：
Hansson, Bill S.
影响因子：
64.8
作者：
Benton, Richard;Vannice, Kirsten S.;Vosshall, Leslie B.
通讯作者：
Vosshall, Leslie B.
影响因子：
14.8
作者：
通讯作者：
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