Reproducible propagation technique for the symbiotic cnidarian model system Cassiopea xamachana.

Reproducible propagation technique for the symbiotic cnidarian model system Cassiopea xamachana.
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DOI:
10.1242/bio.059413
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发表时间:
2022-09-15
期刊:
影响因子:
2.4
通讯作者:
--
中科院分区:
生物学4区
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--
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刺胞动物门由珊瑚、水母、水螅和海葵组成。刺胞动物以其再生能力而闻名,许多物种保持再生完整结构的能力。这种再生能力已经被偶然地用于繁殖目的(通过解剖),用于实验室研究中的一些刺胞动物,但尚未以可重复的方式记录。一个这样的刺胞动物模型系统是钵水母Cassiopea xamachana。C. xamachana已成为研究刺胞动物-藻类共生关系的新兴模式系统,因此确定一种可靠和快速的实验室动物扩增方法至关重要。在这里,我们概述了一种可重复的繁殖方法,用于C的持续繁殖和生长。xamachana息肉。摘要:这份手稿概述了一个解剖协议,以繁殖倒置水母,Cassiopea xamachana。
The phylum Cnidaria is composed of corals, jellyfish, hydras, and sea anemones. Cnidarians are well-known for their regenerative capability, with many species maintaining the ability to regenerate complete structures. This regenerative capacity has been used casually for propagation purposes (via dissection) for some cnidarians used in laboratory research but has yet been documented in a manner meant to be reproducible. One such cnidarian model system is the scyphozoan jellyfish Cassiopea xamachana. C. xamachana has become an emerging model system for studying the cnidarian-algal symbiotic relationship, so determining a reliable and fast method for expansion of laboratory animals is crucial. Here we outline a reproducible propagation method for continued generation and growth of C. xamachana polyps. Summary: This manuscript outlines a dissection protocol to propagate the upside-down jellyfish, Cassiopea xamachana.
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