Molecular Cloning and Analysis of an Acetyl-CoA C-acetyltransferase Gene (EkAACT) from Euphorbia kansui Liou.
Molecular Cloning and Analysis of an Acetyl-CoA C-acetyltransferase Gene (EkAACT) from Euphorbia kansui Liou.
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甘遂大戟乙酰辅酶A C-乙酰转移酶基因 (EkAACT) 的分子克隆与分析
DOI：
10.3390/plants11121539
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发表时间：
2022-06-09
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影响因子：
--
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中科院分区：
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--
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萜类化合物是最大的一类天然产物，对植物细胞功能及其与环境的相互作用至关重要。乙酰辅酶A乙酰转移酶（AACT，EC2.3.1.9）是萜类化合物生物合成的甲羟戊酸途径（MVA）的一个关键起始步骤，受多种内源和外源刺激的调控。本文报道了甘遂中AACT的功能和表达调控活性。与野生型拟南芥相比，EkAACT过表达植株的根长、全苗鲜重和生长形态略有改善。在转基因拟南芥中，MVA途径中AtAACT、AtMDC、AtMK、AtHMGR和AtHMGS的转录水平和总三萜积累显著增加。在NaCl和PEG胁迫下，EkAACT过表达拟南芥的总三萜含量增加，过氧化物酶（POD）和超氧化物歧化酶（SOD）活性提高，根长和幼苗鲜重增加，脯氨酸含量降低，表明植物对非生物胁迫的耐受性增强。因此，AACT作为第一个关键酶，在MVA通路的整体调节中起着重要作用。
Terpenoids are the largest class of natural products and are essential for cell functions in plants and their interactions with the environment. Acetyl-CoA acetyltransferase (AACT, EC2.3.1.9) can catalyze a key initiation step of the mevalonate pathway (MVA) for terpenoid biosynthesis and is modulated by many endogenous and external stimuli. Here, the function and expression regulation activities of AACT in Euphorbia kansui Liou (EkAACT) were reported. Compared with wild-type Arabidopsis, the root length, whole seedling fresh weight and growth morphology of EkAACT-overexpressing plants were slightly improved. The transcription levels of AtAACT, AtMDC, AtMK, AtHMGR, and AtHMGS in the MVA pathway and total triterpenoid accumulation increased significantly in transgenic Arabidopsis. Under NaCl and PEG treatment, EkAACT-overexpressing Arabidopsis showed a higher accumulation of total triterpenoids, higher enzyme activity of peroxidase (POD) and superoxide dismutase (SOD), increased root length and whole seedling fresh weight, and a decrease in the proline content, which indicated that plant tolerance to abiotic stress was enhanced. Thus, AACT, as the first crucial enzyme, plays a major role in the overall regulation of the MVA pathway.
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影响因子：
2.3
作者：
GERSHENZON, J
通讯作者：
GERSHENZON, J
影响因子：
7.2
作者：
Jin, Huanan;Song, Zhihong;Nikolau, Basil J.
通讯作者：
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影响因子：
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作者：
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通讯作者：
Bent, AF
影响因子：
6.4
作者：
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通讯作者：
Swiezewska, Ewa
影响因子：
11.6
作者：
Foyer, CH;Noctor, G
通讯作者：
Noctor, G