Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy.
Isolation and characterization of extracellular vesicles and future directions in diagnosis and therapy.
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DOI：
10.1002/wnan.1835
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发表时间：
2023-01
期刊：
影响因子：
--
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中科院分区：
文献类型：
--
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细胞外囊泡（EV）是由大多数细胞分泌的一类独特且异质的脂质双层纳米颗粒。EV被认为是原核和真核细胞中细胞间通讯的重要介质，因为它们能够将蛋白质、脂质和核酸转移到受体细胞。除了它们的生理作用之外，EV还被认为是病理过程如癌症、传染病和神经退行性疾病的调节剂，为诊断和治疗干预提供了新的潜在靶点。为了全面了解电动汽车作为一种通用细胞生物系统及其转化应用，需要对其进行分离和表征的最佳技术。在这里，我们回顾了这些技术的最新进展，从分离方法到表征技术。随着对EV治疗应用的兴趣不断增长，我们解决了EV相关细胞生物学的基本问题，例如细胞摄取机制及其在组织中的生物分布，以及它们作为药物载体或生物标志物用于微创诊断或作为免疫原的应用所面临的挑战。诊断工具>生物传感治疗方法和药物发现>肿瘤疾病的纳米医学治疗方法和药物发现>传染病的纳米医学这篇文章被分类在：EV分离，标准化和表征的基础研究将带来转化医学诊断和治疗。
Extracellular vesicles (EVs) are a unique and heterogeneous class of lipid bilayer nanoparticles secreted by most cells. EVs are regarded as important mediators of intercellular communication in both prokaryotic and eukaryotic cells due to their ability to transfer proteins, lipids and nucleic acids to recipient cells. In addition to their physiological role, EVs are recognized as modulators in pathological processes such as cancer, infectious diseases, and neurodegenerative disorders, providing new potential targets for diagnosis and therapeutic intervention. For a complete understanding of EVs as a universal cellular biological system and its translational applications, optimal techniques for their isolation and characterization are required. Here, we review recent progress in those techniques, from isolation methods to characterization techniques. With interest in therapeutic applications of EVs growing, we address fundamental points of EV‐related cell biology, such as cellular uptake mechanisms and their biodistribution in tissues as well as challenges to their application as drug carriers or biomarkers for less invasive diagnosis or as immunogens. Diagnostic Tools > Biosensing Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease Therapeutic Approaches and Drug Discovery > Nanomedicine for Infectious Disease This article is categorized under: Fundamental research on EV isolation, standardization and characterisation will bring about translational medical diagnostics and therapy.
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影响因子：
3.9
作者：
通讯作者：
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DOI：
10.1016/j.urolonc.2021.03.010
发表时间：
2021-08-01
影响因子：
2.7
作者：
Bernstein, Darryl Ethan;Piedad, John;Vasdev, Nikhil
通讯作者：
Vasdev, Nikhil
影响因子：
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影响因子：
4.6
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通讯作者：
Stassen FRM
影响因子：
2.8
作者：
Brett, Sabine I.;Lucien, Fabrice;Leong, Hon S.
通讯作者：
Leong, Hon S.