Quantitative trait loci associated with susceptibility to bladder and kidney infections induced by Escherichia coli in female C3H/HeJ mice.
Quantitative trait loci associated with susceptibility to bladder and kidney infections induced by Escherichia coli in female C3H/HeJ mice.
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DOI：
10.1086/595987
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发表时间：
2009-02-01
期刊：
影响因子：
--
通讯作者：
Suhs KA
中科院分区：
文献类型：
--
作者：
Hopkins WJ;Elkahwaji J;Kendziorski C;Moser AR;Briggs PM;Suhs KA
The C3H/HeJ strain of mice develop severe bladder and kidney infections after intravesical inoculation with uropathogenic E. coli. This susceptibility is genetically determined, but the specific genes involved have not been completely defined. The objective of the current study was to use quantitative trait locus (QTL) mapping to identify chromosomal sites in C3H/HeJ mice associated with infection susceptibility. Female mice from a backcross of C3H/HeJ and (BALB/c × C3H/HeJ)F1 mice were inoculated with E. coli, and the number of E. coli present in the bladder and kidneys was quantified ten days later. Genomic DNA was scanned using microsatellite markers to localize chromosomal segments derived from parental strains. Statistical analyses associated infection phenotypes with chromosomal sites. A highly significant QTL for bladder infection susceptibility was identified on chromosome 4, and C3H/HeJ alleles at this locus interacted with BALB/c alleles on chromosome 19 to increase infection severity. A significant QTL on chromosome 6 was associated with severe kidney infections. Increased susceptibility to E. coli bladder and kidney infections in female C3H/HeJ mice is associated with specific chromosomal sites located near genes contributing to host resistance to infection. The results demonstrate the multigenic nature of UTI susceptibility.
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