Construction of a ratiometric two-photon ER-targeting fluorescent probe for the imaging of peroxynitrite in living systems
Construction of a ratiometric two-photon ER-targeting fluorescent probe for the imaging of peroxynitrite in living systems
复制标题
构建用于生命系统中过氧亚硝酸盐成像的比率双光子 ER 靶向荧光探针
DOI：
10.1016/j.snb.2022.132439
复制
发表时间：
2022-07
影响因子：
8.4
通讯作者：
Baoli Dong
中科院分区：
文献类型：
--
作者：
Yaru Sun;Ruifei Wang;Jingxian Wang;Hua Wei;Qingxian Chen;Yue Wang;Baoli Dong
过氧化亚硝酸根（ONOO -）可耗竭内质网内Ca 2+，引起内质网应激，因此精确原位检测ONOO -对研究其生物学作用具有重要意义。在此，我们提出了一个比率双光子ER靶向荧光探针（NAB）检测ONOO -在活细胞和脑组织。NAB利用4-溴甲基苯基硼酸酯作为ONOO -的识别位点，并利用对甲苯磺酰胺作为ER靶向基团。NAB与ONOO -反应后，4-溴甲基苯基硼酸盐转化为羟基，导致荧光颜色由蓝色变为黄色。探针NAB对ONOO -的响应时间为6s，灵敏度高，对ONOO -有很好的选择性.探针NAB主要定位于ER，表现出良好的ER靶向性。比率双光子成像表明，NAB探针可以选择性地检测外源性ONOO -在ER，也可以应用于成像二甲双胍诱导的ONOO -在活的HepG 2细胞。NAB能有效检测脑组织中ONOO -水平的变化，提示抑郁小鼠海马内质网内源性ONOO -水平升高。·开发了一种ONOO -的比率双光子ER靶向探针。·以比率双光子方式对ER处内源性ONOO -的波动进行成像。·ER处的ONOO -水平显示抑郁小鼠海马中的增加。
Peroxynitrite (ONOO - ) could deplete ER-associated Ca 2+ and cause ER stress, and thereby precise and in situ detection of ONOO - at ER is highly important for the study of its biological roles. Herein, we present a ratiometric two-photon ER-targeting fluorescent probe ( NAB ) for the detection of ONOO - in living cells and brain tissues. NAB utilized 4-bromomethylphenyl borate as a recognition site for ONOO - , and utilized p -toluenesulfonamide as ER-targeting group. After the response of NAB to ONOO - , the 4-bromomethylphenyl borate converted to hydroxyl, resulting in the change of the fluorescence color from blue to yellow. The probe NAB could show highly sensitive response to ONOO - within 6 s, and exhibited excellent selectivity to ONOO - . The probe NAB mainly located at ER and showed excellent ER-targeting property. Ratiometric two-photon imaging demonstrated that the probe NAB could selectively detect exogenous ONOO - at ER, and also could be applied to image metformin-induced ONOO - in living HepG2 cells. Furthermore, NAB can effectively detect the changes of ONOO - level in brain tissues, and revealed that the endogenous ONOO - level at ER showed increase in hippocampus of the depressive mouse. • A ratiometric two-photon and ER-targeting probe for ONOO - was developed. • The fluctuation of endogenous ONOO - at ER was imaged in a ratiometric two-photon manner. • ONOO - level at ER showed an increase in hippocampus of the depressive mouse.
登录
查看更多内容
DOI：
10.1073/pnas.1804932115
发表时间：
2018-06-05
影响因子：
11.1
作者：
Radi, Rafael
通讯作者：
Radi, Rafael
影响因子：
3.5
作者：
ISCHIROPOULOS, H;ALMEHDI, AB
通讯作者：
ALMEHDI, AB
DOI：
10.1161/01.atv.0000189159.96900.d9
发表时间：
2005-12-01
影响因子：
8.7
作者：
Dickhout, JG;Hossain, GS;Austin, RC
通讯作者：
Austin, RC
影响因子：
4
作者：
Knewtson KE;Rane D;Peterson BR
通讯作者：
Peterson BR
影响因子：
21.8
作者：
通讯作者：
--