Hormone and RNA-seq analyses reveal the mechanisms underlying differences in seed vigour at different maize ear positions
Hormone and RNA-seq analyses reveal the mechanisms underlying differences in seed vigour at different maize ear positions
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激素和 RNA-seq 分析揭示了不同玉米穗位置种子活力差异的机制
DOI：
10.1007/s11103-019-00830-0
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发表时间：
2019-02
影响因子：
5.1
通讯作者：
Zhang Chunqing
中科院分区：
文献类型：
--
作者：
Wang Mingming;Qv Haibin;Zhang Huidi;Liu Shuai;Li Yan;Zhang Chunqing
关键信息ABA/GA 4比值、抗逆性、碳氮代谢和染色质结构对玉米穗位不同的种子活力差异起着重要作用。然而，对相关机制知之甚少。结果表明，穗中下部种子活力、抗逆性、碳氮代谢均较高，而胚中阿坝/GA 4比值显著较低。与吐丝期重复授粉的种子相比，吐丝完全后单次授粉的不同穗位种子的种子活力和胚中阿坝/GA 4比值变化较小。这说明单花授粉可以减少但不能消除不同穗位种子活力的差异。RNA-seq分析表明，单授粉处理玉米穗不同部位种子活力的差异与碳氮代谢有关。相反，重复授粉下种子活力的差异与染色质结构有关。本研究有助于我们了解玉米穗上不同位置种子活力差异的机制。
Key messageABA/GA4ratio, stress resistance, carbon and nitrogen metabolism, and chromatin structure play important roles in vigour differences of seeds located at different maize ear positions.AbstractSeed vigour, which ensures rapid and uniform field emergence across diverse environments, differs at different maize ear positions. However, little is known regarding the associated mechanisms. In this study, we determined that seed vigour, stress resistance, and carbon and nitrogen metabolism were higher in seeds from middle and bottom section of the ear, while the ABA/GA4ratio in the embryos was significantly lower. Compared with the seeds subjected to repeated pollination during silking, less variation in seed vigour and the ABA/GA4ratio in the embryos was observed in seeds at different ear positions subjected to single pollination after complete silking. This indicated that single pollination can reduce, but not eliminate, the differences in seed vigour at different ear positions. RNA-seq analysis indicated that the seed vigour differences at the different locations of the maize ears of the single pollinated treatment were related to carbon and nitrogen metabolism. In contrast, the differences in seed vigour under repeated pollination were related to chromatin structure. The present study contributes to our understanding of the mechanisms underlying differences in seed vigour at different positions on the maize ear.
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影响因子：
3.4
作者：
Catusse, Julie;Meinhard, Juliane;Job, Dominique
通讯作者：
Job, Dominique
DOI：
10.1016/j.plaphy.2012.07.031
发表时间：
2012-11
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
L. Ventura;M. Donà;A. Macovei;D. Carbonera;A. Buttafava;A. Mondoni;G. Rossi;A. Balestrazzi
通讯作者：
L. Ventura;M. Donà;A. Macovei;D. Carbonera;A. Buttafava;A. Mondoni;G. Rossi;A. Balestrazzi
影响因子：
11.6
作者：
Liu, Yongxiu;Koornneef, Maarten;Soppe, Wim J. J.
通讯作者：
Soppe, Wim J. J.
DOI：
10.1590/s0101-31222005000100002
发表时间：
2005-06
期刊：
Revista Brasileira De Sementes
影响因子：
--
作者：
V. H. V. Mondo-V.-H.-V.-Mondo-87552687;S. M. Cícero
通讯作者：
V. H. V. Mondo-V.-H.-V.-Mondo-87552687;S. M. Cícero
DOI：
10.1111/nph.14053
发表时间：
2016-10
期刊：
The New phytologist
影响因子：
--
作者：
O. Turc;M. Bouteillé;Avan Fuad-Hassan;C. Welcker;F. Tardieu
通讯作者：
O. Turc;M. Bouteillé;Avan Fuad-Hassan;C. Welcker;F. Tardieu