Is hydrogen peroxide an effective mouthwash for reducing the viral load of SARS-CoV-2 in dental clinics?
Is hydrogen peroxide an effective mouthwash for reducing the viral load of SARS-CoV-2 in dental clinics?
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过氧化氢是否是减少牙科诊所中SARS-COV-2病毒负荷的有效漱口水？
DOI：
10.1016/j.sdentj.2022.01.005
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Rodríguez D
中科院分区：
文献类型：
--
作者：
Burgos-Ramos E;Urbieta IR;Rodríguez D
应用RT-PCR方法检测某口腔诊所高效空气过滤器中SARS-CoV-2。在候诊室中检测到SARS-CoV-2，但在治疗室中未检测到。二氧化碳水平表明通风不足和COVID-19传播的风险。证明了H2 O2漱口液的杀病毒作用。先前的研究表明，SARS-CoV-2主要通过吸入气溶胶传播，并可在空气中存活数小时。病毒可以在牙科环境中传播，并使专业人员和患者由于接近和气溶胶生成程序以及空气通风不良而处于感染的高风险中。本研究的目的是研究1%过氧化氢（H2 O2）漱口液对减少口内SARS-CoV-2载量的影响。对暴露3个月的带有HEPA过滤器的便携式空气净化器进行了分析，以测试候诊室（患者戴口罩但不进行漱口）和三个治疗室（患者不戴口罩但进行漱口）中的病毒存在。由于CO2与COVID-19感染者呼出的含有SARS-CoV-2的气溶胶一起，我们还测量了CO2作为室内感染风险的代表。应用特异性引物和探针RT-PCR检测滤膜中SARS-CoV-2病毒基因组。具体来说，我们扩增了SARS-CoV-2的核衣壳基因（Nuclv）。二氧化碳水平介于860至907 ppm之间，因此表明通风不足和COVID-19传播的风险。然而，我们只在候诊室的过滤器中发现了病毒载量，而在治疗室中没有发现。结果显示，使用1%H2O2进行1分钟口腔冲洗的有效性，因为患者在治疗室摘下口罩后立即进行口腔冲洗。我们的研究结果表明，牙科诊所在患者到达诊所后立即用1%H2O2漱口1分钟会更安全，更无COVID-19。
RT-PCR was used to detect SARS-CoV-2 in HEPA filters in a dental clinic. SARS-CoV-2 was detected in the waiting room but not in the treatment rooms. CO2 levels indicated low ventilation and risk of COVID-19 transmission. The virucidal effect of H2O2 mouth rinsing was proven. Previous studies have demonstrated that SARS-CoV-2 is mainly transmitted by inhalation of aerosols and can remain viable in the air for hours. Viruses can spread in dental settings and put professionals and patients at high risk of infection due to proximity and aerosol-generating procedures, and poor air ventilation. The aim of this study was to investigate the effects of a 1% hydrogen peroxide (H2O2) mouth rinse on reducing the intraoral SARS-CoV-2 load. Portable air cleaners with HEPA filters exposed for 3 months were analysed to test for virus presence in a waiting room (where patients wore a face mask but did not undergo mouth rinsing) and three treatment rooms (where patients wore no mask but carried out mouth rinsing). As CO2 is co-exhaled with aerosols containing SARS-CoV-2 by COVID-19 infected people, we also measured CO2 as a proxy of infection risk indoors. Specific primer and probe RT-PCR were applied to detect viral genomes of the SARS-CoV-2 virus in the filters. Specifically, we amplified the nucleocapsid gene (Nuclv) of SARS-CoV-2. CO2 levels ranged from 860 to 907 ppm, thus indicating low ventilation and the risk of COVID-19 transmission. However, we only found viral load in filters from the waiting room and not from the treatment rooms. The results revealed the efficiency of 1-minute mouth rinsing with 1% H2O2 since patients rinsed their mouths immediately after removing their mask in the treatment rooms. Our findings suggest that dental clinics would be safer and more COVID-19 free by implementing mouth rinsing 1 min with 1% H2O2 immediately after the patients arrive at the clinic.
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影响因子：
3.7
作者：
Hammond A;Khalid T;Thornton HV;Woodall CA;Hay AD
通讯作者：
Hay AD
DOI：
10.1016/j.jhin.2020.10.003
发表时间：
2020-12
期刊：
The Journal of hospital infection
影响因子：
--
作者：
Ortega KL;Rech BO;El Haje GLC;Gallo CB;Pérez-Sayáns M;Braz-Silva PH
通讯作者：
Braz-Silva PH
DOI：
10.1016/j.scitotenv.2021.147300
发表时间：
2021-09-01
期刊：
The Science of the total environment
影响因子：
--
作者：
Rodríguez M;Palop ML;Seseña S;Rodríguez A
通讯作者：
Rodríguez A
影响因子：
7.6
作者：
Benzian H;Beltrán-Aguilar E;Mathur MR;Niederman R
通讯作者：
Niederman R
DOI：
10.1111/jopr.13220
发表时间：
2020-07-24
影响因子：
4
作者：
Bidra, Avinash S.;Pelletier, Jesse S.;Tessema, Belachew
通讯作者：
Tessema, Belachew