Behavioral and physiological responses to peripheral injection of flagellin in chicks
Behavioral and physiological responses to peripheral injection of flagellin in chicks
复制标题
雏鸡外周注射鞭毛蛋白的行为和生理反应
DOI：
10.1016/j.physbeh.2021.113433
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发表时间：
2021
影响因子：
2.9
通讯作者：
Cline MA
中科院分区：
文献类型：
--
作者：
Tachibana T;Khan MSI;Makino R;Cline MA
鞭毛蛋白（Flagellin, Flg）是一种在细菌鞭毛中发现的球状蛋白，是一种与病原体相关的分子模式，也是脊椎动物的toll样受体-5 （TLR5）配体。大多数tlr配体参与非特异性效应，如细菌感染动物的厌食和活动不足。然而，人们对Flg对鸟类的影响知之甚少。因此，本研究的目的是确定腹腔注射Flg是否会影响雏鸡（Gallus Gallus）的食物摄取量、自主活动、阴囊温度、嗉囊排空率、血液成分和脾脏细胞因子基因表达。研究了Flg的n端片段Flg22的作用。注射10µg Flg可显著提高雏鸡脾脏白细胞介素-8、干扰素-γ (IFN-γ)和肿瘤坏死因子样细胞因子- 1a基因表达，提示Flg可激活雏鸡先天免疫系统。注射Flg显著降低食物摄取量、自主活动、血糖浓度和作物排空率，并增加肠腔温度和血浆亚硝酸盐、硝酸盐和皮质酮浓度。然而，注射Flg22只影响脾脏中IFN-γ基因的表达，这表明Flg22需要全长才能发挥作用。这些结果表明，TLR5的配体Flg与鸡在细菌感染过程中出现厌食、低活动、体温升高、消化道食物通道紊乱、下丘脑-垂体-肾上腺轴活化等非特异性症状有关。
Flagellin (Flg) is a globular protein, found in bacterial flagella, that serves as a pathogen-associated molecular pattern and also serves as a toll-like receptor-5 (TLR5) ligand in vertebrates. Most ligands for TLRs are involved in non-specific effects such as anorexia and hypoactivity in an animal infected by bacteria. However, there is little knowledge on the effects of Flg in birds. Therefore, the purpose of the present study was to determine if intraperitoneal (IP) injection of Flg affects food intake, voluntary activity, cloacal temperature, crop emptying rate, blood constituents, and splenic gene expression of cytokines in chicks (Gallus gallus). The effect of Flg22, an N-terminus fragment of Flg, was also investigated. IP injection of 10 µg Flg significantly increased the splenic gene expression of interleukin-8, interferon-γ (IFN-γ), and tumor necrosis factor-like cytokine-1A, suggesting that Flg activated the innate immune system in chicks. The injection of Flg significantly decreased food intake, voluntary activity, blood glucose concentration, and crop emptying rate, and increased cloacal temperature and plasma concentrations of nitrite, nitrate, and corticosterone. However, the injection of Flg22 only affected the splenic gene expression of IFN-γ, indicating that the full-length of Flg is required for its action. These results suggest that Flg, a ligand for TLR5, is related to non-specific symptoms including anorexia, hypoactivity, increase in body temperature, disturbance of food passage in the digestive tract, and the activation of hypothalamic-pituitary-adrenal axis during bacterial infection in chicks.
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发表时间：
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