Changes of statistical structural fluctuations unveils an early compacted degraded stage of PNS myelin.
Changes of statistical structural fluctuations unveils an early compacted degraded stage of PNS myelin.
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DOI：
10.1038/srep05430
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发表时间：
2014-06-25
影响因子：
4.6
通讯作者：
Bianconi A
中科院分区：
文献类型：
--
作者：
Poccia N;Campi G;Ricci A;Caporale AS;Di Cola E;Hawkins TA;Bianconi A
髓鞘的降解是从多发性硬化到白质营养不良的脱髓鞘神经系统疾病的常见病理学基础。虽然在沃勒退化的晚期阶段髓鞘超微结构的大畸形是已知的，但在脱髓鞘的早期阶段其微妙的结构变化仍然很难表征。这部分是由于缺乏合适的和非侵入性的实验探针具有足够的分辨率来检测降解。在这里，我们报告的可行性的应用程序的一种创新的非侵入性的局部结构的实验方法成像的统计结构波动的变化，在第一阶段的髓鞘变性。扫描显微X射线衍射，使用同步加速器X射线束聚焦，快速数据收集的进步，与空间统计分析配对，已被用来揭示时间变化的解剖神经的髓鞘结构提取非洲爪蟾坐骨神经。早期髓鞘变性是沃勒氏变性的肿胀髓鞘阶段之前的特定有序的压实阶段。我们证明了使用生物组织进行SµXRD测量的统计分析的可行性，为进一步研究神经元和其他细胞和组织在不同病理状态下的降解和死亡铺平了道路，这些病理状态可能会发现纳米级结构变化。
Degradation of the myelin sheath is a common pathology underlying demyelinating neurological diseases from Multiple Sclerosis to Leukodistrophies. Although large malformations of myelin ultrastructure in the advanced stages of Wallerian degradation is known, its subtle structural variations at early stages of demyelination remains poorly characterized. This is partly due to the lack of suitable and non-invasive experimental probes possessing sufficient resolution to detect the degradation. Here we report the feasibility of the application of an innovative non-invasive local structure experimental approach for imaging the changes of statistical structural fluctuations in the first stage of myelin degeneration. Scanning micro X-ray diffraction, using advances in synchrotron x-ray beam focusing, fast data collection, paired with spatial statistical analysis, has been used to unveil temporal changes in the myelin structure of dissected nerves following extraction of the Xenopus laevis sciatic nerve. The early myelin degeneration is a specific ordered compacted phase preceding the swollen myelin phase of Wallerian degradation. Our demonstration of the feasibility of the statistical analysis of SµXRD measurements using biological tissue paves the way for further structural investigations of degradation and death of neurons and other cells and tissues in diverse pathological states where nanoscale structural changes may be uncovered.
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影响因子：
5.7
作者：
Jensen, T. H.;Bech, M.;Pfeiffer, F.
通讯作者：
Pfeiffer, F.
DOI：
10.1083/jcb.8.1.13
发表时间：
1960-01-01
期刊：
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY
影响因子：
--
作者：
FINEAN, JB
通讯作者：
FINEAN, JB
影响因子：
4
作者：
Albert, R
通讯作者：
Albert, R
影响因子：
3.5
作者：
Cedola, Alessia;Campi, Gaetano;Mastrogiacomo, Maddalena
通讯作者：
Mastrogiacomo, Maddalena
影响因子：
64.8
作者：
Fratini, Michela;Poccia, Nicola;Bianconi, Antonio
通讯作者：
Bianconi, Antonio