Concentration and fate of histatins and acidic proline-rich proteins in the oral environment.
Concentration and fate of histatins and acidic proline-rich proteins in the oral environment.
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DOI：
10.1016/j.archoralbio.2008.11.010
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发表时间：
2009-04
影响因子：
3
通讯作者：
Helmerhorst, Eva J.
中科院分区：
文献类型：
--
作者：
Campese, Melanie;Sun, Xiuli;Bosch, Jos A.;Oppenheim, Frank G.;Helmerhorst, Eva J.
唾液在保护口腔硬组织和软组织中起着至关重要的作用，并且含有大量具有体外生物活性的成分。其中包括组他汀类药物和酸性富含脯氨酸的蛋白质（PRPs）。然而，很少有功能研究认识到这些蛋白在全唾液蛋白水解环境中的结构不稳定性。本研究的目的是测定腮腺分泌物（PS）和全唾液（WS）中的组蛋白和酸性PRP水平，并确定它们对这些唾液中蛋白水解的敏感性。采用阳离子聚丙烯酰胺凝胶电泳和密度分析测定WS总组蛋白（组蛋白1+3+5）平均浓度为33.3±16.7 μg/ml (n=22)，酸性PRP （PRP1/PIF-s+PRP3/PIF-f）平均浓度为427.9±123.3 μg/ml （n=22）。组蛋白和酸性PRP浓度分别是WS的6倍和1.5倍（n=7）。WS组蛋白和酸性PRP水平与WS总蛋白浓度呈极显著相关（P <0.01和P<0.05），且彼此之间呈极显著相关（P <0.01）。组蛋白3和PRP1/Pif-s在PS中的稳定性研究显示，组蛋白3和PRP1/Pif-s分别为7.2±5.5小时和50.3±24.8小时的1.5倍（n=7）。组蛋白3 （40 μg/ml）和PRP1 （400 μg/ml）以与PS相当的浓度添加到WS中，消失速度更快，t½值分别为1.7±1.6 min和29.3±15.3 min （n=7）。数据表明，WS中的蛋白质水解是解释WS中组他汀类药物和酸性PRPs浓度明显低于腺体分泌物的重要因素。
Saliva plays a critical role in the protection of oral hard and soft tissues and contains a multitude of constituents with well-characterized biological activities in vitro. Among these are histatins and acidic proline-rich proteins (PRPs). Nevertheless, few functional studies have recognized the structural instability of these proteins in the proteolytic environment of whole saliva. The aim of this investigation was to determine histatin and acidic PRP levels in parotid secretion (PS) and in whole saliva (WS) as well as to establish their susceptibility to proteolysis in these salivary fluids. Using cationic polyacrylamide gel electrophoresis and densitometric analysis the average total histatin concentration (histatin 1+3+5) in WS was determined to be 33.3 ± 16.7 μg/ml (n=22) and the average total acidic PRP concentration (PRP1/PIF-s+PRP3/PIF-f) was 427.9 ± 123.3 μg/ml (n=22). Histatin and acidic PRP concentrations in PS were 6 and 1.5 times higher than in WS (n=7), respectively. WS histatin and acidic PRP levels each correlated significantly with WS total protein concentrations (P <0.01 and P<0.05, respectively), as well as with each other (P <0.01). Stability studies of histatin 3 and PRP1/Pif-s in PS revealed t½ times of 7.2 ± 5.5 hr and 50.3 ± 24.8 hr, respectively (n=7). Histatin 3 (40 μg/ml) and PRP1 (400 μg/ml), added to WS in concentrations equivalent to their concentrations in PS, disappeared at a much faster rate, with t½ values of 1.7 ± 1.6 min and 29.3 ± 15.3 min, respectively (n=7). The data indicate that proteolysis in WS is an important factor in explaining the substantially lower concentrations of histatins and acidic PRPs in WS as compared to in glandular secretions.
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影响因子：
7
作者：
Messana, Irene;Cabras, Tiziana;Castagnola, Massimo
通讯作者：
Castagnola, Massimo
DOI：
10.1196/annals.1384.030
发表时间：
2007-01-01
期刊：
ORAL-BASED DIAGNOSTICS
影响因子：
--
作者：
Oppenheim, Frank G.;Salih, Erdjan;Helmerhorst, Eva J.
通讯作者：
Helmerhorst, Eva J.
影响因子：
3
作者：
ALHASHIMI, I;LEVINE, MJ
通讯作者：
LEVINE, MJ
影响因子：
4.1
作者：
BRADWAY, SD;BERGEY, EJ;LEVINE, MJ
通讯作者：
LEVINE, MJ
DOI：
10.1177/10454411930040030101
发表时间：
1993-01-01
期刊：
CRITICAL REVIEWS IN ORAL BIOLOGY & MEDICINE
影响因子：
--
作者：
LAMKIN, MS;OPPENHEIM, FG
通讯作者：
OPPENHEIM, FG