Human fetal thymus and bone marrow contain target cells for natural killer cells.
Human fetal thymus and bone marrow contain target cells for natural killer cells.
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人类胎儿胸腺和骨髓含有自然杀伤细胞的靶细胞。
DOI：
10.1002/eji.1830110103
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发表时间：
1981
影响因子：
5.4
通讯作者：
Andersson,B
中科院分区：
文献类型：
--
作者：
Hansson,M;Kiessling,R;Andersson,B
小鼠自然杀伤（NK）系统的先前研究表明，NK细胞可能参与裂解正常的初级造血组织。在本报告中，使用胎儿骨髓（BM）细胞和胸腺细胞以及来自健康供体的成人BM细胞作为靶细胞，在常规的6至12-h51 Cr-释放试验中，在人类NK系统中分析了这一点。成人BM细胞显示出低但显著的敏感性水平，可以通过使用干扰素（IFN）激活的外周血白细胞（PBL）作为效应细胞来增加。来自16至19周龄胎儿的BM细胞始终显示出比成人BM更高的溶解敏感性，并且与先前报道的成人胸腺细胞相比，胎儿胸腺细胞也被证明对溶解非常敏感。当用作针对K562靶标的效应细胞时，成人BM显示出明确的裂解活性，其可以用IFN进一步活化。相比之下，胎儿BM在IFN活化后也完全无NK活性。在健康成人供体中，自体BM与同种异体BM一样有效地裂解，并且当使用不同的NK细胞供体时，无论BM靶的来源如何，都可以看到这些NK高或低反应性细胞的相同分类。干扰素可以增加溶解自体以及在同种异体效应BM靶组合。IFN还可以保护成人骨髓细胞免受NK细胞裂解，但对胎儿骨髓细胞没有保护作用。在尼龙非粘附、E玫瑰花结阴性PBL中观察到针对BM靶的最高NK活性，因此，效应细胞似乎与针对连续细胞系的活性细胞具有相同的性质。这些结果表明，在人NK系统中，NK细胞可以裂解正常BM细胞和胸腺细胞。与成人细胞相比，胎儿BM细胞和胸腺细胞表达的更高敏感性可能表明这些组织中特定原始NK敏感细胞类型的频率增加，这一发现与小鼠NK系统中观察到的结果一致。
Previous studies in the mouse natural killer (NK) system have indicated that NK cells may be involved in lysing normal, primary hematopoietic tissues. In the present report, this was analyzed in the human NK system using fetal bone marrow (BM) cells and thymocytes as well as adult BM cells from healthy donors as target cells in a conventional 6 to 12‐h51Cr‐release assay.Adult BM cells showed low but significant levels of sensitivity, which could be increased by using interferon (IFN)‐activated peripheral blood leukocytes (PBL) as effector cells. BM cells from 16 to 19‐week‐old fetuses consistently showed higher sensitivity for lysis than adult BM, and also fetal thymocytes proved very sensitive for lysis, in contrast to what has previously been reported for adult thymocytes. When used as effector cells against K562 targets, adult BM showed a clear lytic activity which could be further activated with IFN. In contrast, fetal BM was totally NK‐inactive also after IFN activation. Among healthy adult donors, autologous BM was lysed as efficiently as allogeneic BM, and when different NK cell donors were used, the same classification of these as NK high or low reactive cells was seen regardless of the source of BM targets. IFN could augment lysis in autologous as well as in allogeneic effector BM target combinations. IFN could also protect adult BM cells from NK lysis, but no protection was seen with fetal BM cells. The highest NK activity against BM targets was seen among nylon‐nonadherent, E rosette‐negative PBL and, therefore, the effector cell seemed to be of the same nature as that active against continuous cell lines.These results show that in the human NK system, NK cells can lyse normal BM cells and thymocytes. The higher sensitivity expressed by fetal BM cells and thymocytes as compared to adult cells may suggest an increased frequency of a particular primitive NK‐sensitive cell type in these tissues, a finding which is in line with results seen in the mouse NK system.
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DOI：
10.1084/jem.177.1.19
发表时间：
1993-01-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Sánchez MJ;Gutiérrez-Ramos JC;Fernández E;Leonardo E;Lozano J;Martínez C;Toribio ML
通讯作者：
Toribio ML
影响因子：
64.8
作者：
WU, L;SCOLLAY, R;SHORTMAN, K
通讯作者：
SHORTMAN, K
影响因子：
2.2
作者：
YSSEL, H;DEVRIES, JE;SPITS, H
通讯作者：
SPITS, H
人胸腺器官培养物中胎肝 CD34 谱系细胞的淋巴和骨髓分化
影响因子：
15.3
作者：
A. Bárcena;A. Galy;J. Punnonen;M. Muench;D. Schols;M. Roncarolo;J. D. de Vries;H. Spits
通讯作者：
H. Spits
人类胎儿肝脏中免疫系统的发育。
DOI：
10.1007/978-94-009-3365-1_6
发表时间：
1987
期刊：
Thymus
影响因子：
--
作者：
R. Gale
通讯作者：
R. Gale