Resistance exercise increases leg muscle protein synthesis and mTOR signalling independent of sex.
Resistance exercise increases leg muscle protein synthesis and mTOR signalling independent of sex.
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DOI：
10.1111/j.1748-1716.2010.02074.x
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发表时间：
2010-05
期刊：
影响因子：
--
通讯作者：
Rasmussen BB
中科院分区：
文献类型：
--
作者：
Dreyer HC;Fujita S;Glynn EL;Drummond MJ;Volpi E;Rasmussen BB
人类骨骼肌的性别差异很明显，因为男性个体肌肉纤维的横截面积比女性更大。我们最近的研究表明，在运动后恢复的早期阶段，阻力运动可以刺激人体的mTOR信号和肌肉蛋白质合成。因此，这项研究的目的是确定性别是否影响阻力运动恢复过程中的肌肉蛋白质合成反应。17名受试者，9名男性和8名女性，在高强度腿部阻力运动前、中和之后的2小时内进行了禁食状态的研究。用稳定同位素技术测定混合肌肉蛋白合成率(FSR)，用免疫印迹法检测重复股外侧肌活检标本的mTOR信号。运动后，男性肌肉蛋白质合成增加了52%，女性增加了47%(P<；0.05)，但组间没有差异(P>；0.05)。两组AKT磷酸化在运动后1小时升高(P<；0.05)，运动后2小时恢复至基线水平，组间无差异(P>；0.05)。运动后恢复期mTOR及其下游效应蛋白S6K1的磷酸化水平显著升高，组间差异无统计学意义(P<；0.05)。在运动后1小时和2小时，两组的eEF2磷酸化降低程度相似(P<；0.05)。收缩引起的运动后早期mTOR信号和肌肉蛋白质合成的增加与性别无关，似乎没有在年轻男性和女性腿部骨骼肌性二形性中发挥作用。
Sex differences are evident in human skeletal muscle as the cross-sectional area of individual muscle fibres is greater in men as compared to women. We have recently shown that resistance exercise stimulates mTOR signalling and muscle protein synthesis in humans during early post-exercise recovery. Therefore, the aim of this study was to determine if sex influences the muscle protein synthesis response during recovery from resistance exercise. Seventeen subjects, 9 male and 8 female, were studied in the fasted state before, during and for two hours following a bout of high-intensity leg resistance exercise. Mixed muscle protein fractional synthetic rate (FSR) was measured using stable isotope techniques and mTOR signalling was assessed by immunoblotting from repeated vastus lateralis muscle biopsy samples. Post-exercise muscle protein synthesis increased by 52% in the men and by 47% in the women (P<0.05) and was not different between groups (P>0.05). Akt phosphorylation increased in both groups at 1 hr post-exercise (P<0.05) and returned to baseline during 2 hr post-exercise with no differences between groups (P>0.05). Phosphorylation of mTOR and its downstream effector S6K1 increased significantly and similarly between groups during post-exercise recovery (P<0.05). eEF2 phosphorylation decreased at 1- and 2 hrs post-exercise (P<0.05) to a similar extent in both groups. The contraction-induced increase in early post-exercise mTOR signalling and muscle protein synthesis is independent of sex and appears to not be playing a role in the sexual dimorphism of leg skeletal muscle in young men and women.
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发表时间：
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