Electrochemical sensing of cortisol: a recent update.
Electrochemical sensing of cortisol: a recent update.
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DOI：
10.1007/s12010-014-0894-2
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发表时间：
2014-10
影响因子：
3
通讯作者：
Bhansali, Shekhar
中科院分区：
文献类型：
--
作者：
Singh, Aparajita;Kaushik, Ajeet;Kumar, Rajesh;Nair, Madhavan;Bhansali, Shekhar
日常生活方式引起的心理压力会导致个体的健康差异。它影响许多生物标志物，但皮质醇“一种类固醇激素”被认为是心理压力检测的潜在生物标志物。皮质醇水平异常表明患有库欣综合征、艾迪生病、肾上腺功能不全以及最近的创伤后应激障碍 (PTSD) 等疾病。传统上用于检测皮质醇的色谱技术是一个需要多步骤提取/纯化的复杂系统。这限制了其在皮质醇即时检测 (POC) 中的应用。然而，皮质醇的电化学免疫传感是 POC 应用的最新进展。本综述重点介绍了最近开发的简单、低成本、无标记的电化学免疫传感平台，用于灵敏、选择性地检测生物液中的皮质醇。电化学检测用于使用共价固定在自组装单层 (SAM)、聚合物复合材料等纳米结构上的抗皮质醇抗体 (Anti-Cab) 来检测皮质醇，用于传感器的 POC 集成。观察到的信息可以用作原型来了解人类（例如农民、消防员等）的行为变化。佛罗里达国际大学生物MEMS和微系统研究小组牢记未来的方向和挑战，重点是开发用于免疫传感的POC设备、将这些设备与微流体集成、与现有技术进行交叉验证以及对真实样本进行分析。
Psychological stress caused by everyday life style contributes to health disparities experience by individuals. It affects many biomarkers, but cortisol “a steroid hormone” is known as a potential biomarker for psychological stress detection. Abnormal levels of cortisol, is indicative of conditions such as Cushing’s syndrome Addison’s disease, adrenal insufficiencies and more recently post-traumatic stress disorder (PTSD). Chromatographic techniques, which are traditionally used to detect cortisol, are a complex system requiring multistep extraction/purification. This limits its application for point-of-care (POC) detection of cortisol. However, electrochemical immunosensing of cortisol is a recent advancement towards POC application. This review highlights simple, low-cost, and label-free electrochemical immunosensing platforms which have been developed recently for sensitive and selective detection of cortisol in biofluids. Electrochemical detection is utilized for the detection of cortisol using Anti-Cortisol antibodies (Anti-Cab) covalently immobilized on nanostructures such as self-assembled monolayer (SAM), polymer composite, etc. for POC integration of sensors. The observed information can be used as prototype to understand behavioral changes in humans in case to case such as farmers, fire fighters, etc. Keeping the future directions and challenges in mind the focus of the BioMEMS and Microsystems Research Group at Florida International University is on development of POC devices for immunosensing, integration of these devices with microfluidics, cross validation with existing technologies, and analysis of real sample.
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