Immunofluorescence Assay of Ablated Colorectal Liver Metastases: The Frozen Section of Image-Guided Tumor Ablation?
Immunofluorescence Assay of Ablated Colorectal Liver Metastases: The Frozen Section of Image-Guided Tumor Ablation?
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DOI：
10.1016/j.jvir.2021.11.008
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发表时间：
2022-03
影响因子：
2.9
通讯作者：
Sofocleous, Constantinos T.
中科院分区：
文献类型：
--
作者：
Kamarinos, Nikiforos Vasiniotis;Vakiani, Efsevia;Fujisawa, Sho;Gonen, Mithat;Fan, Ning;Romin, Yevgeniy;Do, Richard K. G.;Ziv, Etay;Erinjeri, Joseph P.;Petre, Elena N.;Sotirchos, Vlasios S.;Camacho, Juan C.;Solomon, Stephen B.;Manova-Todorova, Katia;Sofocleous, Constantinos T.
验证免疫荧光试验（IFA）检测残留存活肿瘤作为术中热消融（TA）区域评估，并证明其对结直肠肝转移（CLM）TA后局部肿瘤进展的预后价值。这项前瞻性、IRB批准的研究纳入了2009年11月至2019年1月期间消融的99例患者，共消融了155个CLM。消融后30分钟内，对消融区（AZ）中心和最小边缘的组织样本进行免疫荧光显微镜检查，询问细胞形态和线粒体活力（IFA）。随后用标准形态学和免疫组织化学（IHC）方法评价相同的组织样本。计算IFA与标准形态学和IHC检查的灵敏度、特异性和总体准确性。评估12个月随访期的局部肿瘤无进展（LTP）生存率。在311份染色的组织样本中，304份（98%）被视为可评价。27%（81/304）的标本被认为存在活肿瘤。IFA诊断符合率为94%（286/304）。敏感性为100%（63/63），特异性为93%（223/241）。18个假阳性IFA评估对应于包括活胆管细胞的样本。IFA阳性和阴性的肿瘤AZs的12个月无LTP生存率分别为59%和78%（P <0.001）。仅边缘阳性与中央AZ阳性肿瘤之间的LTP没有差异（25% vs 31%，p=1）。AZ的IFA评估可在术中完成，并可作为完全肿瘤根除或检测TA后残留存活肿瘤的有效实时生物标志物。这种方法可以提高TA对肿瘤的控制。
To validate an immuno-fluorescent assay (IFA) detecting residual viable tumor as intraprocedural thermal ablation (TA) zone assessment and demonstrate its prognostic value for local tumor progression after colorectal liver metastases (CLM) TA. This prospective, IRB-approved study included 99 patients with 155 CLMs ablated between November 2009 and January 2019. Tissue samples from the ablation zone (AZ) center and minimal margin underwent immunofluorescent microscopic examination interrogating cellular morphology and mitochondrial viability (IFA) within 30 minutes after ablation. The same tissue samples were subsequently evaluated with standard morphological and immunohistochemical (IHC) methods. Sensitivity, specificity, and overall accuracy of IFA versus standard morphological and IHC examination were calculated. Local tumor progression (LTP)-free survival rates were evaluated for 12-month follow-up period. Of the 311 tissue samples stained, 304 (98%) were deemed evaluable. 27% (81/304) of specimens were considered positive for the presence of viable tumor. The accuracy of IFA was 94% (286/304). Sensitivity and specificity were 100% (63/63) and 93% (223/241), respectively. The 18 false-positive IFA assessments corresponded to samples that included viable cholangiocytes. The 12-month LTP-free survival was 59% vs 78% for IFA positive vs negative for viable tumor AZs, respectively (P <.001). There was no difference in LTP between margin positive only versus central AZ positive tumors (25% vs 31%, p=1). IFA assessment of the AZ can be completed intra-procedurally and serve as a valid real-time biomarker of complete tumor eradication or detect residual viable tumor after TA. This method could improve tumor control by TA.
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影响因子：
5.2
作者：
Dijkstra M;Nieuwenhuizen S;Puijk RS;Timmer FEF;Geboers B;Schouten EAC;Opperman J;Scheffer HJ;Vries JJJ;Swijnenburg RJ;Versteeg KS;Lissenberg-Witte BI;van den Tol MP;Meijerink MR
通讯作者：
Meijerink MR
影响因子：
4.7
作者：
Sandu RM;Paolucci I;Ruiter SJS;Sznitman R;de Jong KP;Freedman J;Weber S;Tinguely P
通讯作者：
Tinguely P
影响因子：
19.7
作者：
Shyn, Paul B.;Casadaban, Leigh C.;Silverman, Stuart G.
通讯作者：
Silverman, Stuart G.
影响因子：
3.7
作者：
Sofocleous CT;Garg S;Petrovic LM;Gonen M;Petre EN;Klimstra DS;Solomon SB;Brown KT;Brody LA;Covey AM;Dematteo RP;Schwartz L;Kemeny NE
通讯作者：
Kemeny NE
影响因子：
5.9
作者：
Calandri, Marco;Yamashita, Suguru;Odisio, Bruno C.
通讯作者：
Odisio, Bruno C.