Elucidation of host diversity of the VanD-carrying genomic islands in enterococci and anaerobes.
Elucidation of host diversity of the VanD-carrying genomic islands in enterococci and anaerobes.
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DOI：
10.1093/jacamr/dlab189
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发表时间：
2022-03
影响因子：
3.4
通讯作者：
Tomita H
中科院分区：
文献类型：
--
作者：
Hashimoto Y;Hisatsune J;Suzuki M;Kurushima J;Nomura T;Hirakawa H;Kojima N;Ono Y;Hasegawa Y;Tanimoto K;Sugai M;Tomita H
VAND是一种全球罕见的万古霉素耐药类型。然而，Vand基因簇的宿主多样性及其基因组岛的结构相似性还不是很清楚。对从2017年接受万古霉素治疗的日本患者分离的3株Vand型粪肠球菌(AA620、AA622和AA624)进行了分析。本研究利用WGS分析对三株Vand型粪肠球菌进行了特征分析，并描述了具有Vand携带基因组岛的宿主的多样性。这三个分离株对万古霉素的MIC值不同。在对万古霉素相对耐药的AA620中，发现了VanSD和DDL的突变。菌株AA622和AA624具有完整的DDL，并含有两个Vand基因簇。QRT-PCR结果显示，DDL突变是影响AA620对万古霉素高耐药范围的一个因素。WGS数据显示，含有Vand基因簇的155kb和185kb基因组岛插入到Lyss基因的编码区，分别位于AA620和AA622/624的染色体上。将携带Vand的基因组岛与其他肠球菌和肠道厌氧菌的可用序列进行比较，揭示了世界各地分离的这些生物的基因组岛如何共享相似的核心基因和骨架。这些厌氧菌分属于真细菌目的各个属。基因组岛核心基因组序列的系统发育簇与寄主-物种谱系无关，表明肠道微生物区系中的水平基因转移。通过水平基因转移，形成肠道微生物区系的各种细菌维持携带Vand的基因组岛。
VanD is a rare type of vancomycin resistance worldwide. However, the host diversity of the vanD gene cluster and the structural similarity of their genomic islands are not well understood. Three VanD-type Enterococcus faecium strains (AA620, AA622 and AA624) isolated from a Japanese patient who underwent vancomycin treatment in 2017 were analysed. This study utilized WGS analysis to characterize the three VanD-type E. faecium strains and describes the diversity of hosts possessing VanD-carrying genomic islands. The three isolates exhibited variable MICs of vancomycin. In the relatively vancomycin-resistant AA620, mutations were identified in vanSD and ddl. The strains AA622 and AA624 had intact ddl and harboured two vanD gene clusters. qRT-PCR results revealed the ddl mutation to be a factor affecting the high vancomycin resistance range of AA620. WGS data showed the 155 kb and 185 kb genomic islands harbouring the vanD gene cluster inserted in the coding region of the lysS gene, located in the chromosome in AA620 and AA622/624, respectively. Comparing the VanD-carrying genomic islands to available sequences of other enterococci and enteric anaerobes revealed how the genomic islands of these organisms isolated worldwide shared similar core genes and backbones. These anaerobes belonged to various genera within the order Eubacteriales. The phylogenetic cluster of the genomic island core genome alignment did not correlate with the host-species lineage, indicating horizontal gene transfer in the gut microbiota. By horizontal gene transfer, various bacteria forming the gut microbiota maintain VanD-carrying genomic islands.
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影响因子：
9.4
作者：
Lavigne, JP;Marchandin, H;Sotto, A
通讯作者：
Sotto, A
影响因子：
4.6
作者：
Flipse, Jacky;von Wintersdorf, Christian J. H.;van Alphen, Lieke B.
通讯作者：
van Alphen, Lieke B.
影响因子：
4.9
作者：
Domingo, MC;Huletsky, A;Bergeron, MG
通讯作者：
Bergeron, MG
影响因子：
5.2
作者：
Sato, Toyotaka;Wada, Takayuki;Yokota, Shin-ichi
通讯作者：
Yokota, Shin-ichi
DOI：
10.1099/ijsem.0.001143
发表时间：
2016-08-01
影响因子：
2.8
作者：
Shkoporov, Andrei N.;Chaplin, Andrei V.;Efimov, Boris A.
通讯作者：
Efimov, Boris A.