A novel calmodulin-like protein gene in rice which has an unusual prolonged C-terminal sequence carrying a putative prenylation site.
A novel calmodulin-like protein gene in rice which has an unusual prolonged C-terminal sequence carrying a putative prenylation site.
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水稻中的一种新型钙调蛋白样蛋白基因，其具有不寻常的长C端序列，带有推定的异戊二烯化位点。
DOI：
10.1093/dnares/6.3.179
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
XIAO, C., XIN, H., DONG, A., SUN, C. , CAO K.,
中科院分区：
文献类型：
--
作者：
XIAO, C., XIN, H., DONG, A., SUN, C. , CAO K.,
鉴定了一个编码新型钙调素样蛋白的水稻cDNA。它在149个氨基酸的完整的典型钙调蛋白（CaM）序列的C-末端具有38个额外的氨基酸。四个C-末端氨基酸残基形成CAAL基序，其可能是蛋白质异戊二烯化的位点，并且随后可能导致蛋白质成为膜结合的。RT-PCR分析证实，这种组合蛋白基因确实存在于水稻中。其基因组序列分析表明，有一个内含子位于连接正常的CaM序列和38个C-末端氨基酸。如果涉及选择性剪接机制，这将为正常CaM引入潜在的终止密码子。对水稻基因组DNA的Southern杂交分析表明，该基因只有一个位点。北方杂交分析表明，该基因在水稻根、茎和花中均有高表达。这种蛋白的分布证明了这种新型钙调素样蛋白在水稻中的功能重要性。
A rice cDNA encoding a novel calmodulin-like protein was identified. It has 38 additional amino acids at the C-terminus of a complete, typical calmodulin (CaM) sequence of 149 amino acids. The four C-terminal amino acid residues form a CAAL motif which could be a site for protein prenylation and may subsequently cause the protein to become membrane associated. RT-PCR analysis confirmed that such a combined protein gene truly exists in rice. Sequence analysis of its genomic counterpart showed that there is an intron located at junction of the normal CaM sequence and the 38 C-terminal amino acids. This introduces a potential stop codon for normal CaM if an alternative splicing mechanism is involved. Southern blot analysis of rice genomic DNA revealed that there is only one locus for this gene. The northern blot analysis showed that this gene is highly expressed in rice roots, shoots and flowers. The distribution of this protein demonstrates the functional importance of this novel CaM-like protein in rice.
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影响因子：
64.5
作者：
BRAAM, J;DAVIS, RW
通讯作者：
DAVIS, RW
影响因子：
3.5
作者：
Y. Saimi;C. Kung
通讯作者：
Y. Saimi;C. Kung
影响因子：
4.9
作者：
Daye Sun;Y. Bian;Baoshan Zhao;Liangxia Zhao;Xian-Ming Yu;Shengjun Duan
通讯作者：
Daye Sun;Y. Bian;Baoshan Zhao;Liangxia Zhao;Xian-Ming Yu;Shengjun Duan
DOI：
10.1016/s0021-9258(19)50616-0
发表时间：
1992-02
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
B. Kinsella;W. Maltese
通讯作者：
B. Kinsella;W. Maltese
DOI：
--
发表时间：
1990
期刊：
Advances in second messenger and phosphoprotein research
影响因子：
--
作者：
A. Nairn
通讯作者：
A. Nairn