Bioconjugate of lysozyme and the antibacterial marine sesquiterpene quinone avarone and its derivatives.
Bioconjugate of lysozyme and the antibacterial marine sesquiterpene quinone avarone and its derivatives.
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DOI：
10.1021/bc200330m
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发表时间：
2012-01-18
影响因子：
4.7
通讯作者：
Sladic, Dusan
中科院分区：
文献类型：
--
作者：
Novakovic, Irena;Andelkovic, Uros;Zlatovic, Mario;Gasic, Miroslav J.;Sladic, Dusan
合成了一种溶菌酶与海洋生物活性倍半萜醌阿凡酮及其三个衍生物的缀合物。MALDI TOF质谱分析和胰蛋白酶消化分析表明，所有衍生物修饰的溶菌酶残基只有赖氨酸97。残基的身份与分子模型预测的结果完全相关。所有的生物偶联物都保留了溶菌酶的大部分酶活性。与溶菌酶相比，偶联物的熔点略有增加，表明结构略有稳定。所有结合物对革兰氏阳性菌和革兰氏阴性菌的抑菌活性均强于溶菌酶和醌类菌的抑菌活性，部分结合物的MIC值在低微摩尔范围内。
A conjugate of lysozyme with avarone, a bioactive sesquiterpene quinone of marine origin, and its three derivatives, was synthesized. MALDI TOF mass spectral analysis and tryptic digestion showed that the only residue in lysozyme that was modified by all derivatives was lysine 97. The identity of the residue was in full correlation with the prediction obtained by molecular modelling. All bioconjugates preserved most of the enzymatic activity of lysozyme. The melting point of the conjugates was slightly increased in comparison to lysozyme, indicating a slight stabilization of structure. The antibacterial activity of all the conjugates to both Gram positive and Gram negative bacteria was stronger than the activity of either lysozyme or the quinones, the MIC values being in low micromolar range for some conjugates.
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影响因子：
1
作者：
Novakovic, I;Vujcic, Z;Sladic, D
通讯作者：
Sladic, D
影响因子：
2.9
作者：
Matters, Dominic;Cooper, Helen J.;Peterson, Ian
通讯作者：
Peterson, Ian
影响因子：
3.3
作者：
Cegielska-Radziejewska, Renata;Lesnierowski, Grzegorz;Kijowski, Jacek
通讯作者：
Kijowski, Jacek
DOI：
10.1107/s0907444908030503
发表时间：
2008-12-01
影响因子：
2.2
作者：
Cianci, Michele;Helliwell, John R.;Suzuki, Atsuo
通讯作者：
Suzuki, Atsuo
DOI：
10.1016/0006-3002(52)90045-0
发表时间：
1952-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
SHUGAR, D
通讯作者：
SHUGAR, D