Modulation of JNK-1/ β-catenin signaling by Lactobacillus casei, inulin and their combination in 1,2-dimethylhydrazine-induced colon cancer in mice.
Modulation of JNK-1/ β-catenin signaling by Lactobacillus casei, inulin and their combination in 1,2-dimethylhydrazine-induced colon cancer in mice.
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DOI：
10.1039/c9ra04388h
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发表时间：
2019-09-13
期刊：
影响因子：
3.9
通讯作者：
Kandeil, Mohamed A.
中科院分区：
文献类型：
--
作者：
Ali, Mohammed S.;Hussein, Rasha M.;Gaber, Yasser;Hammam, Olfat A.;Kandeil, Mohamed A.
结肠癌是一种复杂的疾病，涉及许多基因改变，使正常的结肠粘膜转变为侵袭性腺癌。本研究评价了菊粉（益生元）、干酪乳杆菌（Lactobacillus casei，益生菌）及其联合（synbiotic）对1,2-二甲基肼（DMH）诱导的雄性瑞士小鼠结肠癌的保护作用。动物分为：对照组、DMH处理组、DMH加菊糖组、DMH加酪蛋白乳杆菌组和DMH加菊糖加酪蛋白乳杆菌处理组。进行粪便微生物组分析、生化测定、结肠组织病理学检查、免疫染色和Western blotting分析β-catenin、GSK3β和JNK-1。与dmh组相比，益生元组、益生菌组和合成菌组的癌胚抗原水平降低，异常隐窝灶数量减少，其中合成菌组的效果更佳。此外，所有治疗均显示出减肥效果。菊粉、干酪乳杆菌或两者联合施用可提高磷酸化jnk -1的表达水平，降低磷酸化β-catenin和磷酸化gsk3 β的表达水平。值得注意的是，干酪乳杆菌处理导致某些有益菌属如Akkermansia和Turicibacter的富集。因此，干酪乳杆菌和菊粉作为一种合成组合对小鼠结肠癌有保护作用。干酪乳杆菌和菊粉可调节JNK-1、GSK3β和β-catenin蛋白的表达，丰富有益菌群，对小鼠结肠癌有保护作用。
Colon cancer is a complex disease that involves numerous genetic alterations that change the normal colonic mucosa into invasive adenocarcinoma. In the current study, the protective effects of inulin (prebiotic), Lactobacillus casei (L. casei, probiotic) and their combination (synbiotic) on 1,2-dimethylhydrazine (DMH)-induced colon cancer in male Swiss mice were evaluated. Animals were divided into: Control group, DMH-treated group, DMH plus inulin, DMH plus L. casei and DMH plus inulin plus L. casei-treated groups. Fecal microbiome analysis, biochemical measurements, histopathological examination of the colon tissues, immunostaining and Western blotting analysis of β-catenin, GSK3β and JNK-1 were performed. The prebiotic-, probiotic- and synbiotic-treated groups showed decreased levels of carcinoembryonic antigen and a lower number of aberrant crypt foci compared to the DMH-treated group with the synbiotic group exhibiting a superior effect. Furthermore, all treatments showed a body weight-reducing effect. Administration of inulin, L. casei or their combination increased the expression level of phospho-JNK-1 while they decreased the expression level of β-catenin and phospho-GSK3β. Remarkably, L. casei treatment resulted in enrichment of certain beneficial bacterial genera i.e. Akkermansia and Turicibacter. Therefore, administration of L. casei and inulin as a synbiotic combination protects against colon cancer in mice. The lactobacillus casei and inulin modulate the expression of JNK-1, GSK3β and β-catenin proteins and enrich the beneficial bacteria to protect from colon cancer in mice.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
DOI：
10.1073/pnas.1219451110
发表时间：
2013-05-28
影响因子：
11.1
作者：
Everard, Amandine;Belzer, Clara;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
影响因子：
4.7
作者：
Hu, Dong;Fang, Wenfeng;Yang, Wancai
通讯作者：
Yang, Wancai
DOI：
10.1590/2175-8239-jbn-3931
发表时间：
2018-09-01
期刊：
Brazilian Journal of Nephrology
影响因子：
--
作者：
Fagundes, Raquel Aparecida Bandeira;Soder, Taís Fátima;Mendes, Roberta Hack
通讯作者：
Mendes, Roberta Hack