Structural insights into a unique preference for 3' terminal guanine of mirtron in Drosophila TUTase tailor.
Structural insights into a unique preference for 3' terminal guanine of mirtron in Drosophila TUTase tailor.
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果蝇 TUTase 裁缝中对 mirtron 3 英寸末端鸟嘌呤的独特偏好的结构见解
DOI：
10.1093/nar/gky1116
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发表时间：
2019-01-10
影响因子：
14.9
通讯作者：
Gong Q
中科院分区：
文献类型：
--
作者：
Cheng L;Li F;Jiang Y;Yu H;Xie C;Shi Y;Gong Q
末端尿苷酰转移酶（TUTase）是一种通过促进转录后添加尿苷酰核糖核苷酸到RNA分子3′端来修饰RNA分子的酶。最近的研究报道，果蝇TUTase，Tailor，表现出对以3′G结尾的RNA底物的内在偏好，使其与其他已知的TUTases区别开来。通过这种独特的功能，Tailor在剪接衍生的mirtron pre-miRNA的生物发生途径中起着至关重要的作用。本文描述了Tailor核心催化结构域及其与RNA链段5′-AGU-3′和5′-AGUU-3′复合物的晶体结构。我们发现R327和N347是Tailor偏爱3′G的两个关键残基，R327可能在促进多聚尿苷化链的延伸中发挥额外的作用。我们还表明，构象稳定性的RNA结合沟的出口也有助于显着的Tailor的活动。总之，我们的工作揭示了果蝇Tailor优先选择以3′G结尾的RNA底物的原因，并为进一步理解果蝇mirtron生物合成途径的生物学意义提供了重要的参考价值。
Terminal uridylyl transferase (TUTase) is one type of enzyme that modifies RNA molecules by facilitating the post-transcriptional addition of uridyl ribonucleotides to their 3′ ends. Recent researches have reported that Drosophila TUTase, Tailor, exhibits an intrinsic preference for RNA substrates ending in 3′G, distinguishing it from any other known TUTases. Through this unique feature, Tailor plays a crucial role as the repressor in the biogenesis pathway of splicing-derived mirtron pre-miRNAs. Here we describe crystal structures of core catalytic domain of Tailor and its complexes with RNA stretches 5′-AGU-3′ and 5′-AGUU-3′. We demonstrate that R327 and N347 are two key residues contributing cooperatively to Tailor's preference for 3′G, and R327 may play an extra role in facilitating the extension of polyuridylation chain. We also demonstrate that conformational stability of the exit of RNA-binding groove also contributes significantly to Tailor's activity. Overall, our work reveals useful insights to explain why Drosophila Tailor can preferentially select RNA substrates ending in 3′G and provides important values for further understanding the biological significances of biogenesis pathway of mirtron in flies.
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DOI：
10.1073/pnas.0912632107
发表时间：
2010-02-23
影响因子：
11.1
作者：
Ibrahim, Fadia;Rymarquis, Linda A.;Cerutti, Heriberto
通讯作者：
Cerutti, Heriberto
影响因子：
3.5
作者：
Aphasizhev, R;Aphasizheva, I;Simpson, L
通讯作者：
Simpson, L
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
7
作者：
Ladewig E;Okamura K;Flynt AS;Westholm JO;Lai EC
通讯作者：
Lai EC
DOI：
10.1261/rna.058107.116
发表时间：
2016-11
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Lackey PE;Welch JD;Marzluff WF
通讯作者：
Marzluff WF