Maternal vitamin D deficiency during pregnancy affects expression of adipogenic-regulating genes peroxisome proliferator-activated receptor gamma (PPARγ) and vitamin D receptor (VDR) in lean male mice offspring.
Maternal vitamin D deficiency during pregnancy affects expression of adipogenic-regulating genes peroxisome proliferator-activated receptor gamma (PPARγ) and vitamin D receptor (VDR) in lean male mice offspring.
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DOI：
10.1007/s00394-016-1359-x
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发表时间：
2018-03
影响因子：
5
通讯作者：
Peterson CA
中科院分区：
文献类型：
--
作者：
Belenchia AM;Jones KL;Will M;Beversdorf DQ;Vieira-Potter V;Rosenfeld CS;Peterson CA
孕期母体维生素D缺乏是一个普遍存在的问题，可能对后代的肥胖状况产生长期影响。我们试图确定围产期母体维生素D缺乏会如何影响后代脂肪组织的发育和基因表达。
雌性C57BL/6J小鼠从怀孕前4周（受孕前后）到产后7天，分别喂食维生素D缺乏（VDD）饮食或对照饮食。雄性后代在75日龄（成年早期）称重并处死，此时收集血清进行生化分析，切除性腺周围和皮下白色脂肪组织（分别为PGAT和SQAT），称重，然后迅速冷冻，以便后续进行组织学研究和生脂基因表达分析。
所有成年雄性后代均未肥胖；在体重、脂肪垫重量或脂肪细胞大小方面没有显著差异。然而，暴露于VDD的后代，其生脂调节基因过氧化物酶体增殖物激活受体γ（Pparg）和维生素D受体（Vdr）的表达更高。
这项研究表明，围产期暴露于维生素D缺乏可直接影响非肥胖后代脂肪组织发育相关基因。这些新发现促使进一步研究孕期母体维生素D状况影响后代脂肪发育和代谢健康的机制。
Maternal vitamin D deficiency during pregnancy is a widespread issue that may have long-lasting consequences on offspring adiposity. We sought to determine how maternal vitamin D deficiency during the perinatal period would affect offspring adipose tissue development and gene expression. Female C57BL/6 J mice were fed either a vitamin D deficient (VDD) or control diet from 4 weeks before pregnancy (periconception) until 7 days postparturition. Male offspring were weighed and euthanized at 75 days of age (early adult period), at which point serum was collected for biochemical analyses, and perigonadal and subcutaneous white adipose tissue (PGAT and SQAT, respectively) were excised, weighed, then flash-frozen for later histology and analyses of adipogenic gene expression. All adult male offspring were nonobese; there were no significant differences in body weight, adipose pad weight, or adipocyte size. However, VDD-exposed offspring had greater expression of the adipogenic-regulating genes peroxisome proliferator-activated receptor gamma (Pparg) and vitamin D receptor (Vdr). This study suggests that exposure to vitamin D deficiency during the perinatal period can directly affect genes involved in the development of adipose tissue in non-obese offspring. These novel findings invite further investigation into the mechanisms by which maternal vitamin D status during pregnancy affects adipose development and metabolic health of offspring.
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影响因子：
2
作者：
Johnson, Donna D.;Wagner, Carol L.;Hollis, Bruce W.
通讯作者：
Hollis, Bruce W.
影响因子：
9.8
作者：
Cho, Geum Joon;Hong, Soon-Cheol;Kim, Hai-Joong
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Kim, Hai-Joong
影响因子：
5.6
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Jung UJ;Choi MS
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Choi MS
影响因子：
4.8
作者：
BELLOWS, CG;WANG, YH;AUBIN, JE
通讯作者：
AUBIN, JE
影响因子：
2.8
作者：
Ballesteros-Tato A
通讯作者：
Ballesteros-Tato A