Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells.
Super-Resolution Microscopy and FIB-SEM Imaging Reveal Parental Centriole-Derived, Hybrid Cilium in Mammalian Multiciliated Cells.
复制标题
DOI：
10.1016/j.devcel.2020.09.016
复制
发表时间：
2020-10-26
影响因子：
11.8
通讯作者：
Mennella V
中科院分区：
文献类型：
--
作者：
Liu Z;Nguyen QPH;Nanjundappa R;Delgehyr N;Megherbi A;Doherty R;Thompson J;Jackson C;Albulescu A;Heng YM;Lucas JS;Dell SD;Meunier A;Czymmek K;Mahjoub MR;Mennella V
运动纤毛是细胞跳动机器，在粘液纤毛清除、脑脊液运动和生育中起关键作用。在呼吸道中，存在于多纤毛上皮细胞表面的数百个运动纤毛协调地跳动以保护上皮免受细菌、病毒和有害颗粒的侵害。在多纤毛细胞分化过程中，活动纤毛是从基底体中模板化的，每个基底体延伸一个基底足，基底足是将活动纤毛连接在一起以确保协调跳动的附属物。在这里，我们表明，在许多能动纤毛的multicilated细胞，一个混合纤毛的结构特征的初级和能动纤毛是窝藏。哺乳动物多纤毛细胞中的杂种纤毛是保守的，起源于亲本中心粒，其细胞位置是偏向的，并依赖于纤毛的跳动。我们进一步表明，杂交纤毛出现独立于其他运动纤毛，并在调节基体对齐的功能。Liu，Nguyen等人发现，在多纤毛细胞的数百个纤毛中，有一个纤毛在结构、起源和细胞位置上与其他纤毛不同，它参与了基体排列。
Motile cilia are cellular beating machines that play a critical role in mucociliary clearance, cerebrospinal fluid movement and fertility. In the airways, the hundreds of motile cilia present on the surface of a multiciliated epithelia cell beat coordinately to protect the epithelium from bacteria, viruses and harmful particulates. During multiciliated cell differentiation, motile cilia are templated from basal bodies each extending a basal foot, an appendage linking motile cilia together to ensure coordinated beating. Here, we demonstrate that among the many motile cilia of a multiciliated cell, a hybrid cilium with structural features of both primary and motile cilia is harboured. The hybrid cilium is conserved in mammalian multiciliated cells, originates from parental centrioles and its cellular position is biased and dependent on ciliary beating. We further show that the hybrid cilium emerges independently of other motile cilia, and functions in regulating basal body alignment. Liu, Nguyen et al. show that among hundreds of cilia of a multiciliated cell, there is one that differs from the others in structure, origin and cellular position, which participates in basal body alignment.
登录
查看更多内容
影响因子：
64.8
作者：
Al Jord, Adel;Lemaitre, Anne-Iris;Meunier, Alice
通讯作者：
Meunier, Alice
DOI：
10.1165/rcmb.2014-0483oc
发表时间：
2015-10-01
影响因子：
6.4
作者：
Frommer, Adrien;Hjeij, Rim;Omran, Heymut
通讯作者：
Omran, Heymut
影响因子：
4.6
作者：
Lu, Hao;Anujan, Priyanka;Roy, Sudipto
通讯作者：
Roy, Sudipto
基底脚MTOC组织了击败纤毛的协调所需的支柱。
影响因子：
16.6
作者：
Clare DK;Magescas J;Piolot T;Dumoux M;Vesque C;Pichard E;Dang T;Duvauchelle B;Poirier F;Delacour D
通讯作者：
Delacour D
影响因子：
7.7
作者：
Chong, Weng Man;Wang, Won-Jing;Liao, Jung-Chi
通讯作者：
Liao, Jung-Chi