Identification and functional characterization of three new terpene synthase genes involved in chemical defense and abiotic stresses in Santalum album
Identification and functional characterization of three new terpene synthase genes involved in chemical defense and abiotic stresses in Santalum album
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檀香中参与化学防御和非生物胁迫的三个新萜烯合酶基因的鉴定和功能表征
DOI：
10.1186/s12870-019-1720-3
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发表时间：
2019-03
影响因子：
5.3
通讯作者：
Guohua Ma
中科院分区：
文献类型：
--
作者：
Xinhua Zhang;Meiyun Niu;Jaime A. Teixeira da Silva;Yueya Zhang;Yunfei Yuan;Yongxia Jia;Yangyang Xiao;Yuan Li;Lin Fang;Songjun Zeng;Guohua Ma
研究背景：檀香心材中提取的芳香精油是一种重要的植物精油。具有广泛的经济价值。结果：在本研究中，在成熟的檀香叶片中检测到了α-檀香烯、表-β-檀香烯、β-檀香烯、α-檀香醇、β-檀香醇、（E）-α-香柠檬烯、（E）-β-法尼烯和β-红没药烯等挥发性化合物。相册树我们通过挖掘可公开获得的RNA-seq数据鉴定了40多个候选萜合酶（TPS）unigenes，并表征了由三种cDNA编码的酶：一种单-TPS催化大部分α-萜品醇的形成，和两种多官能倍半-TPS，其中一种产生（E）-α-香柠檬烯和倍半卡宾烯作为主要产物，另一种催化（E）-β-法呢烯、（E）-橙花叔醇和（E，E）-法尼醇为主要产品。代谢特征和基因表达研究证实，檀香醇含量与檀香烯合成酶（SaSSY）基因转录本密切相关，SaSSY是檀香醇合成的关键酶。然而，三个新的SaTPS基因的表达差异显着SaSSY在精油生产心材。超氧化物歧化酶、过氧化氢酶、过氧化物酶和抗坏血酸过氧化物酶等抗氧化酶的活性在不同组织中均有不同程度的提高。在施用ImM茉莉酸甲酯（MeJA）和ImM水杨酸（SA）或暴露于4°C、38°C和高光强度之后，对植物进行处理。MeJA和SA显著诱导叶片中SaTPS 1和SaTPS 2的表达。不同组织中的SaTPS 1至3转录本在逆境温度和光照胁迫下被差异激活。与此相反，几乎所有的SaSSY转录本在这些环境胁迫下都有所下降，不像SaTPS 1至3。结论：檀香中存在多功能酶，包括一个叶绿体单TPS和两个胞质倍半TPS。我们的研究结果表明，这三个新的SaTPS基因在防御反应的生物攻击和非生物胁迫的生态重要性。专辑
Background:It is well known that aromatic essential oils extracted from the heartwood of Santalum album L. have wide economic value. However, little is known about the role of terpenoids in response to various adverse environmental stresses as other plants do in the form of signals during plant-environment interactions.Results:In this study, trace amounts of volatiles consisting of α-santalene, epi-β-santalene, β-santalene, α-santalol, β-santalol, (E)-α-bergamotene, (E)-β-farnesene and β-bisabolene were found in the leaves of mature S. album trees. We identified more than 40 candidate terpene synthase (TPS) unigenes by mining publicly-available RNA-seq data and characterized the enzymes encoded by three cDNAs: one mono-TPS catalyzes the formation of mostly α-terpineol, and two multifunctional sesqui-TPSs, one of which produces (E)-α-bergamotene and sesquisabinene as major products and another which catalyzes the formation of (E)-β-farnesene, (E)-nerolidol and (E,E)-farnesol as main products. Metabolite signatures and gene expression studies confirmed that santalol content is closely related with santalene synthase (SaSSY) transcripts in heartwood, which is key enzyme responsible for santalol biosynthesis. However, the expression of three new SaTPS genes differed significantly from SaSSY in the essential oil-producing heartwood. Increased activities of antioxidant enzymes, superoxide dismutase, catalase, peroxidase and ascorbate peroxidase, were detected in different tissues of S. album plants after applying 1 mM methyl jasmonate (MeJA) and 1 mM salicylic acid (SA), or exposure to 4°C, 38°C and high light intensity. MeJA and SA dramatically induced the expression of SaTPS1 and SaTPS2 in leaves. SaTPS1 to 3 transcripts were differentially activated among different tissues under adverse temperature and light stresses. In contrast, almost all SaSSY transcripts decreased in response to these environmental stresses, unlike SaTPS1 to 3.Conclusions:Multifunctional enzymes were biochemically characterized, including one chloroplastic mono-TPS and two cytosolic sesqui-TPSs in sandalwood. Our results suggest the ecological importance of these three new SaTPS genes in defensive response to biotic attack and abiotic stresses in S. album.
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DOI：
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发表时间：
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