Non-canonical Phototransduction Mediates Synchronization of the Drosophila melanogaster Circadian Clock and Retinal Light Responses.
Non-canonical Phototransduction Mediates Synchronization of the Drosophila melanogaster Circadian Clock and Retinal Light Responses.
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DOI：
10.1016/j.cub.2018.04.016
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发表时间：
2018-06-04
期刊：
影响因子：
--
通讯作者：
Stanewsky R
中科院分区：
文献类型：
--
作者：
Ogueta M;Hardie RC;Stanewsky R
每天的光暗周期是同步生物钟的关键信号。昆虫和哺乳动物都有专门的“昼夜节律”光感受器，但也利用视觉系统来重置时钟。在果蝇中，生物钟重置是由蓝光光感受器隐色素（CRY）实现的，它在大脑时钟神经元的亚群中表达。此外，表达视紫红质的光感受器细胞也参与光同步。尽管在关键的昼夜节律起搏器神经元的特定亚群中，包括小腹侧神经元（s-LNvs）， TIM和周期（PER）振荡可以通过独立于CRY和典型视紫红质光导的光来同步，但光通过依赖于CRY的时钟蛋白降解来重置分子钟。本研究表明，果蝇的7种视紫红质中至少有3种可以利用与异源三聚体G蛋白相同的α-亚基在典型视觉光传导（Gq）中操作的替代转导机制。令人惊讶的是，在缺乏无受体电位A （norpA）基因编码的典型磷脂酶C-β (PLC-β)的突变体中，我们发现了一种使用Plc21C基因编码的不同PLC-β的新的转导途径。这种新途径对于行为时钟重置到半自然的光暗周期很重要，并介导s-LNv时钟神经元内的光依赖分子同步。复眼中与norpa无关的光反应似乎也是同样的途径。我们发现，存在于视网膜感光细胞R8亚群中的视紫红质5 （Rh5）和Rh6驱动眼睛的长期昼夜节律和快速光反应。在没有norpA的情况下，Plc21C介导这种新的光输入，Rhodopsin 1、Rhodopsin 5和Rhodopsin 6可以通过这种依赖gq的途径发出信号，在norpA突变体中，同样的途径介导R8光感受器的ERG反应。果蝇生物钟的光同步依赖于隐色素和Rhodopsin光感受器。Ogueta等人发现，视紫红质向生物钟发出的信号涉及非规范的光传导，其同步关键的昼夜节律起搏器神经元和行为。在眼睛中，同样的通路可以介导ERG对短暂光脉冲的反应。
The daily light-dark cycles represent a key signal for synchronizing circadian clocks. Both insects and mammals possess dedicated “circadian” photoreceptors but also utilize the visual system for clock resetting. In Drosophila, circadian clock resetting is achieved by the blue-light photoreceptor cryptochrome (CRY), which is expressed within subsets of the brain clock neurons. In addition, rhodopsin-expressing photoreceptor cells contribute to light synchronization. Light resets the molecular clock by CRY-dependent degradation of the clock protein Timeless (TIM), although in specific subsets of key circadian pacemaker neurons, including the small ventral lateral neurons (s-LNvs), TIM and Period (PER) oscillations can be synchronized by light independent of CRY and canonical visual Rhodopsin phototransduction. Here, we show that at least three of the seven Drosophila rhodopsins can utilize an alternative transduction mechanism involving the same α-subunit of the heterotrimeric G protein operating in canonical visual phototransduction (Gq). Surprisingly, in mutants lacking the canonical phospholipase C-β (PLC-β) encoded by the no receptor potential A (norpA) gene, we uncovered a novel transduction pathway using a different PLC-β encoded by the Plc21C gene. This novel pathway is important for behavioral clock resetting to semi-natural light-dark cycles and mediates light-dependent molecular synchronization within the s-LNv clock neurons. The same pathway appears to be responsible for norpA-independent light responses in the compound eye. We show that Rhodopsin 5 (Rh5) and Rh6, present in the R8 subset of retinal photoreceptor cells, drive both the long-term circadian and rapid light responses in the eye. CRYPTOCHROME-independent light input synchronizes key circadian pacemaker neurons In the absence of norpA, Plc21C mediates this novel light input Rhodopsin 1, Rhodopsin 5, and Rhodopsin 6 can signal via this Gq-dependent pathway The same pathway mediates ERG responses from R8 photoreceptors in norpA mutants Light synchronization of the Drosophila circadian clock relies on cryptochrome and rhodopsin photoreceptors. Ogueta et al. reveal that rhodopsin signaling to the clock involves non-canonical phototransduction, which synchronizes key circadian pacemaker neurons and behavior. In the eye, the same pathway can mediate ERG responses to brief light pulses.
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影响因子：
56.9
作者：
Koh, Kyunghee;Zheng, Xiangzhong;Sehgal, Amita
通讯作者：
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影响因子：
64.8
作者：
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DOI：
10.1073/pnas.1212317110
发表时间：
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影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1083/jcb.201603076
发表时间：
2016-10-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chaix A;Zarrinpar A;Panda S
通讯作者：
Panda S