Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors.

Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors.
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DOI:
10.1038/s41588-023-01486-9
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发表时间:
2023-09
期刊:
影响因子:
30.8
通讯作者:
Jones, Jonathan D. G.
Jones, Jonathan D. G.
中科院分区:
生物学1区
文献类型:
--
作者:
Lin, Xiao;Jia, Yuxin;Heal, Robert;Prokchorchik, Maxim;Sindalovskaya, Maria;Olave-Achury, Andrea;Makechemu, Moffat;Fairhead, Sebastian;Noureen, Azka;Heo, Jung;Witek, Kamil;Smoker, Matthew;Taylor, Jodie;Shrestha, Ram-Krishna;Lee, Yoonyoung;Zhang, Chunzhi;Park, Soon Ju;Sohn, Kee Hoon;Huang, Sanwen;Jones, Jonathan D. G.

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马铃薯(Solanum tuberosum)和番茄(Solanum lycopersicon)作物遭受由卵菌病原体致病疫霉(Phytophthora infestans)引起的晚疫病的严重损失。美洲茄(Solanumamericanum)是马铃薯和番茄的近缘种,在世界范围内广泛分布,且大多数种质具有高度的抗枯萎病性。我们生成了四个S的高质量参考基因组。americanum材料,对52份材料进行了重新测序,确定了S. americanum免疫受体基因。我们进一步筛选了315 P识别的变化。infestans RXLR效应子52个。美洲的加入。使用这些基因组和表型数据,我们克隆了识别同源致病疫霉RXLR效应子PITG_22825(AVRamr 4)、PITG_02860和PITG_04373的三个NLR编码基因Rpi-amr 4、R 02860和R 04373。这些基因组资源和方法将支持工程马铃薯持久抗晚疫病的努力,并可应用于其他作物的疾病。高质量的基因组组装的四个Solanum Americanum加入导致识别三个NLR编码基因,Rpi-amr 4,R 02860和R 04373,识别马铃薯晚疫病病原体致病疫霉效应子。
Potato (Solanum tuberosum) and tomato (Solanum lycopersicon) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans. Solanum americanum, a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated high-quality reference genomes of four S. americanum accessions, resequenced 52 accessions, and defined a pan-NLRome of S. americanum immune receptor genes. We further screened for variation in recognition of 315P. infestans RXLR effectors in 52 S. americanum accessions. Using these genomic and phenotypic data, we cloned three NLR-encoding genes, Rpi-amr4, R02860 and R04373, that recognize cognate P. infestans RXLR effectors PITG_22825 (AVRamr4), PITG_02860 and PITG_04373. These genomic resources and methodologies will support efforts to engineer potatoes with durable late blight resistance and can be applied to diseases of other crops. High-quality genome assemblies of four Solanum Americanum accessions lead to the identification of three NLR-encoding genes, Rpi-amr4, R02860 and R04373, that recognize potato late blight pathogen Phytophthora infestans effectors.
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