Polyarginine molecular weight determines transfection efficiency of calcium condensed complexes.
Polyarginine molecular weight determines transfection efficiency of calcium condensed complexes.
复制标题
DOI：
10.1021/mp3007117
复制
发表时间：
2013-05-06
影响因子：
4.9
通讯作者：
Berkland CJ
中科院分区：
文献类型：
--
作者：
Alhakamy NA;Berkland CJ
Cell penetrating peptides (CPPs) have been extensively studied in polyelectrolyte complexes as a means to enhance the transfection efficiency of plasmid DNA (pDNA). Increasing the molecular weight of CPPs often enhances gene expression, but poses a risk of increased cytotoxicity and immunogenicity compared to low molecular weight CCPs. Conversely, low molecular weight CPPs typically have low transfection efficiency due to large complex size. Complexes made using low molecular weight CPPs were found to be condensed to a small size by adding calcium. In this study, complexes of low molecular weight polyarginine and pDNA were condensed with calcium. These complexes showed high transfection efficiency and low cytotoxicity in A549 carcinomic human alveolar basal epithelial cells. The relationship between transfection efficiency and polyarginine size (5, 7, 9 or 11 amino acids), polyarginine/pDNA charge ratios, and calcium concentrations were studied. Polyarginine 7 was significantly more effective than other polyarginines under most formulation conditions suggesting a link between cell penetration ability and transfection efficiency.
登录
查看更多内容
DOI：
10.1093/database/bas015
发表时间：
2012
期刊：
Database : the journal of biological databases and curation
影响因子：
--
作者：
Gautam A;Singh H;Tyagi A;Chaudhary K;Kumar R;Kapoor P;Raghava GP
通讯作者：
Raghava GP
影响因子：
12.4
作者：
Crombez, Laurence;Aldrian-Herrada, Gudrun;Divita, Gilles
通讯作者：
Divita, Gilles
影响因子：
10.8
作者：
de Raad, Markus;Teunissen, Erik A.;Mastrobattista, Enrico
通讯作者：
Mastrobattista, Enrico
影响因子：
5.3
作者：
Davidson, TJ;Harel, S;Troy, CM
通讯作者：
Troy, CM
影响因子：
--
作者：
Chiu, YL;Ali, A;Rana, TM
通讯作者：
Rana, TM