Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance.
Genome-Wide Analysis of NF-Y Genes in Potato and Functional Identification of StNF-YC9 in Drought Tolerance.
复制标题
马铃薯 NF-Y 基因的全基因组分析及 StNF-YC9 耐旱功能鉴定
DOI：
10.3389/fpls.2021.749688
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Si H
中科院分区：
文献类型：
--
作者：
Li S;Zhang N;Zhu X;Ma R;Liu S;Wang X;Yang J;Si H
核因子Y （NF-Y）家族由参与多种植物生物学过程的转录因子组成。然而，人们对马铃薯中的这个家族知之甚少。本研究共在马铃薯基因组中鉴定出41个StNF-Y基因。此外，对该家族的系统发育、基因结构、基序和染色体定位进行了分析。基于RNA-seq数据的组织表达谱显示，27个StNF-Y基因具有组织特异性表达，其余14个在所有组织中均低表达。来自各种非生物胁迫的公开转录组学数据揭示了几个应激响应的StNF-Y基因，并通过定量实时聚合酶链反应实验进一步验证。此外，StNF-YC9基因受脱水和干旱处理的诱导程度较高。StNF-YC9蛋白主要定位于细胞核和细胞质膜。过表达StNF-YC9的马铃薯株系（OxStNF-YC9）在聚乙二醇和脱落酸处理下，根系长度显著增加，气孔关闭能力增强。此外，与野生型相比，OxStNF-YC9系在短期干旱胁迫下具有更高的光合速率和更少的水分流失量。在长期干旱胁迫下，OxStNF-YC9系脯氨酸水平升高，丙二醛含量降低，超氧化物歧化酶、过氧化氢酶和过氧化物酶等几种抗氧化酶活性升高。本研究增加了我们对StNF-Y基因的认识，并表明StNF-YC9通过提高马铃薯的光合速率、抗氧化酶活性和脯氨酸积累以及降低丙二醛积累，在耐旱性中发挥重要作用。
The nuclear factor Y (NF-Y) family is comprised of transcription factors that have been implicated in multiple plant biological processes. However, little is known about this family in potato. In the present study, a total of 41 StNF-Y genes were identified in the potato genome. In addition, the phylogenetic, gene structure, motif, and chromosomal location of this family were analyzed. The tissue expression profiles based on RNA-seq data showed that 27 StNF-Y genes had tissue-specific expression, while the remaining 14 had low expression in all tissues. Publicly available transcriptomics data from various abiotic stresses revealed several stress-responsive StNF-Y genes, which were further verified via quantitative real-time polymerase chain reaction experiments. Furthermore, the StNF-YC9 gene was highly induced by dehydration and drought treatments. StNF-YC9 protein was mainly localized in the nucleus and cytoplasmic membrane. Overexpressing StNF-YC9 potato lines (OxStNF-YC9) had significantly increased in root length and exhibited stronger stomatal closure in potato treated by polyethylene-glycol and abscisic acid. In addition, OxStNF-YC9 lines had higher photosynthetic rates and decreased water loss under short-term drought stress compared to wild-type plants. During long-term drought stress, OxStNF-YC9 lines had higher proline levels, lower malondialdehyde content, and increased activity of several antioxidant enzymes, including superoxide dismutase, catalase, and peroxidase. This study increased our understanding of the StNF-Y gene and suggested that StNF-YC9 played an important role in drought tolerance by increased the photosynthesis rate, antioxidant enzyme activity, and proline accumulation coupled to lowered malondialdehyde accumulation in potato.
登录
查看更多内容
影响因子：
5.6
作者：
Griffiths CA;Gaff DF;Neale AD
通讯作者：
Neale AD
影响因子：
3.7
作者：
Leyva-González MA;Ibarra-Laclette E;Cruz-Ramírez A;Herrera-Estrella L
通讯作者：
Herrera-Estrella L
影响因子：
5.2
作者：
Lee, Dong-Keun;Kim, Hyung Il;Kim, Ju-Kon
通讯作者：
Kim, Ju-Kon
影响因子：
7.4
作者：
JOHNSONFLANAGAN, AM;OWENS, JN
通讯作者：
OWENS, JN
影响因子：
5.3
作者：
Kahle, J;Baake, M;Albig, W
通讯作者：
Albig, W