Spatial and temporal control of targeting Polo-like kinase during meiotic prophase.
Spatial and temporal control of targeting Polo-like kinase during meiotic prophase.
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DOI：
10.1083/jcb.202006094
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发表时间：
2020-11-02
期刊：
影响因子：
--
通讯作者：
Kim Y
中科院分区：
文献类型：
--
作者：
Brandt JN;Hussey KA;Kim Y
Brandt等建立了在C.优雅CDK-1磷酸化联会复合体组分SYP-1，以产生PLK-2的对接位点，其缔合被阻止直到交叉形成以确保同源配对、联会和染色体重塑。Polo样激酶（PLKs）在减数分裂染色体动力学中起着广泛保守的作用。然而，PLK如何在减数分裂过程中靶向不同的亚细胞定位仍然知之甚少。在这里，我们证明了细胞周期蛋白依赖性激酶CDK-1通过C.优雅SYP-1被CDK-1磷酸化发生在减数分裂开始之前。然而，PLK-2与SC的对接被核质HAL-2/3复合物阻止，直到交叉指定，这将PLK-2限制在称为配对中心的特殊染色体区域，以确保适当的同源配对和突触。PLK-2靶向由CDK-1引发的交叉位点，并且仅当产生阈值水平的交叉时，通过在每个交叉的一侧上增强SYP-1磷酸化而沿着SC扩散。因此，染色体自主信号传导和全核交叉计数机制的整合相对于交叉位点划分了全着丝粒染色体，这最终定义了减数分裂I期间染色体分离的模式。
Brandt et al. establish mechanisms that target Polo-like kinase during meiotic prophase in C. elegans. CDK-1 phosphorylates a synaptonemal complex component, SYP-1, to generate docking sites for PLK-2, whose association is prevented until crossover formation to ensure homologue pairing, synapsis, and chromosome remodeling. Polo-like kinases (PLKs) play widely conserved roles in orchestrating meiotic chromosome dynamics. However, how PLKs are targeted to distinct subcellular localizations during meiotic progression remains poorly understood. Here, we demonstrate that the cyclin-dependent kinase CDK-1 primes the recruitment of PLK-2 to the synaptonemal complex (SC) through phosphorylation of SYP-1 in C. elegans. SYP-1 phosphorylation by CDK-1 occurs just before meiotic onset. However, PLK-2 docking to the SC is prevented by the nucleoplasmic HAL-2/3 complex until crossover designation, which constrains PLK-2 to special chromosomal regions known as pairing centers to ensure proper homologue pairing and synapsis. PLK-2 is targeted to crossover sites primed by CDK-1 and spreads along the SC by reinforcing SYP-1 phosphorylation on one side of each crossover only when threshold levels of crossovers are generated. Thus, the integration of chromosome-autonomous signaling and a nucleus-wide crossover-counting mechanism partitions holocentric chromosomes relative to the crossover site, which ultimately defines the pattern of chromosome segregation during meiosis I.
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