Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation.
Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation.
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DOI：
10.1155/2017/8749751
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发表时间：
2017
影响因子：
4.3
通讯作者：
Nishimura N
中科院分区：
文献类型：
--
作者：
Iwatani S;Shono A;Yoshida M;Yamana K;Thwin KKM;Kuroda J;Kurokawa D;Koda T;Nishida K;Ikuta T;Fujioka K;Mizobuchi M;Taniguchi-Ikeda M;Morioka I;Iijima K;Nishimura N
间充质干细胞(MSCs)是一种异质细胞群，最初从骨髓(BM)中分离出来，随后几乎从所有组织中分离出来，包括脐带(UC)。UC来源的MSCs(UC-MSCs)由于其旺盛的增殖和分化能力，作为治疗各种退行性疾病的细胞来源而受到越来越多的关注。尽管已知骨髓来源的MSCs的细胞增殖和分化随着年龄的增长而下降，但早产儿和足月儿UC-MSCs之间的功能差异尚不清楚。在本研究中，我们从23名孕22-40周出生的婴儿中分离出UC-MSCs，并分析其基因表达和细胞增殖情况。基因芯片分析显示早产期UC-MSCs的整体基因表达与足月期UC-MSCs不同。WNT信号影响多种组织干细胞的增殖和分化，其通路基因在早产儿和足月儿UC-MSCs之间的差异表达基因中得到丰富。与足月期UC-MSCs相比，早产UC-MSCs的细胞增殖显著增强，并被WNT信号抑制剂XAV939抑制。此外，UC-MSCs中WNT2B的表达与胎龄呈显著负相关。这些结果提示，WNT信号参与了GA依赖的UC-MSC增殖的调节。
Mesenchymal stem cells (MSCs) are a heterogeneous cell population that is isolated initially from the bone marrow (BM) and subsequently almost all tissues including umbilical cord (UC). UC-derived MSCs (UC-MSCs) have attracted an increasing attention as a source for cell therapy against various degenerative diseases due to their vigorous proliferation and differentiation. Although the cell proliferation and differentiation of BM-derived MSCs is known to decline with age, the functional difference between preterm and term UC-MSCs is poorly characterized. In the present study, we isolated UC-MSCs from 23 infants delivered at 22–40 weeks of gestation and analyzed their gene expression and cell proliferation. Microarray analysis revealed that global gene expression in preterm UC-MSCs was distinct from term UC-MSCs. WNT signaling impacts on a variety of tissue stem cell proliferation and differentiation, and its pathway genes were enriched in differentially expressed genes between preterm and term UC-MSCs. Cell proliferation of preterm UC-MSCs was significantly enhanced compared to term UC-MSCs and counteracted by WNT signaling inhibitor XAV939. Furthermore, WNT2B expression in UC-MSCs showed a significant negative correlation with gestational age (GA). These results suggest that WNT signaling is involved in the regulation of GA-dependent UC-MSC proliferation.
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影响因子：
7.5
作者：
Batsali AK;Pontikoglou C;Koutroulakis D;Pavlaki KI;Damianaki A;Mavroudi I;Alpantaki K;Kouvidi E;Kontakis G;Papadaki HA
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影响因子：
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作者：
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CAPLAN, AI