Cellular and molecular characteristics of pollen abortion in chrysanthemum cv. Kingfisher
Cellular and molecular characteristics of pollen abortion in chrysanthemum cv. Kingfisher
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菊花花粉败育的细胞和分子特征。
DOI：
10.1007/s11103-018-0777-y
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发表时间：
2018-09
影响因子：
5.1
通讯作者：
Teng NIanjun
中科院分区：
文献类型：
--
作者：
Wang Fan;Zhong Xinhua;Huang Lulu;Fang Weimin;Chen Fadi;Teng NIanjun
四分体时期的小孢子退化与绒毡层退化迟缓有关。一些与细胞衰老和死亡有关的基因和蛋白质是花粉败育的关键因素。摘要菊花（Chrysanthemum morifolium）是世界上主要的花卉作物，但花粉污染是菊花生产中亟待解决的问题。翠鸟是一种成熟花药中不含花粉的菊花品种，是培育无花粉污染菊花的重要材料。但其花粉败育的机制尚不清楚。本研究利用透射电子显微镜、RNA测序、等压标记相对定量和绝对定量以及生物信息学等手段对‘翠鸟’花粉败育的细胞和分子机制进行了研究。四分体期前小孢子母细胞减数分裂正常，四分体期小孢子开始退化，四分体期后花药中未见小孢子。此外，转录组学和蛋白质组学分析表明，一些与细胞衰老和死亡相关的基因和蛋白质与菊花花粉败育有关。这些结果表明，四分体时期是花粉败育的主要时期，与细胞衰老和死亡相关的基因和蛋白质对花粉败育起着重要作用。这些结果增加了我们对菊花花粉败育的认识，对今后开发无花粉污染的花有一定的帮助。
Key messageMicrospore degeneration at the tetrad stage is associated with tapetum degeneration retardation. Some genes and proteins related to cell senescence and death are the key factors for pollen abortion.AbstractChrysanthemum (Chrysanthemum morifolium) is a major floriculture crop in the world, but pollen contamination is an urgent problem to be solved in chrysanthemum production.C. morifolium‘Kingfisher’ is a chrysanthemum cultivar that does not contain any pollen in mature anthers, thus it is a very important material for developing chrysanthemum without pollen contamination. However, the mechanism of its pollen abortion remains unclear. In this study, the cellular and molecular mechanisms of ‘Kingfisher’ pollen abortion were investigated using transmission electron microscopy, RNA sequencing, isobaric tags for relative and absolute quantitation, and bioinformatics. It was found that the meiosis of microspore mother cells was normal before the tetrad stage, the microspores began to degenerate at the tetrad stage, and no microspores were observed in the anthers after the tetrad stage. In addition, transcriptomic and proteomic analyses showed that some genes and proteins related to cell senescence and death were identified to be implicated in chrysanthemum pollen abortion. These results indicated that the tetrad stage was the main period of pollen abortion, and the genes and proteins related to cell senescence and death contributed to pollen abortion. These add to our understanding of chrysanthemum pollen abortion and will be helpful for development of flowers without pollen contamination in the future.
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影响因子：
46.9
作者：
Trapnell C;Williams BA;Pertea G;Mortazavi A;Kwan G;van Baren MJ;Salzberg SL;Wold BJ;Pachter L
通讯作者：
Pachter L
影响因子：
4.4
作者：
Omidvar V;Mohorianu I;Dalmay T;Fellner M
通讯作者：
Fellner M
DOI：
10.1073/pnas.0912333107
发表时间：
2010-03-30
影响因子：
11.1
作者：
Ye, Qianqian;Zhu, Wenjiao;Wang, Xuelu
通讯作者：
Wang, Xuelu
影响因子：
1.1
作者：
Sheoran, Inder S.;Sawhney, Vipen K.
通讯作者：
Sawhney, Vipen K.
影响因子：
7.4
作者：
Chunsheng Zhang;Kim Norris-Caneda;W. Rottmann;J. Gulledge;Shujun Chang;Brian Kwan;Anita Thomas;Lydia C. Mandel;R. T. Kothera;A. D. Victor;L. Pearson;M. Hinchee
通讯作者：
Chunsheng Zhang;Kim Norris-Caneda;W. Rottmann;J. Gulledge;Shujun Chang;Brian Kwan;Anita Thomas;Lydia C. Mandel;R. T. Kothera;A. D. Victor;L. Pearson;M. Hinchee